ArticleThe Journal of clinical investigation1997
Ventilation and oxygenation induce endothelial nitric oxide synthase gene expression in the lungs of fetal lambs.
Article in The Journal of clinical investigation, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
16 citing papers in PubMed, 98 citations in OpenAlex.
- Impact of Nitric Oxide Metabolites On Early Perioperative Biochemical Changes and Postoperative Complications in High-BMI Patients Undergoing On-Pump CABG: an Exploratory Observational Cohort Study.Cardiovascular drugs and therapy · 2026Article
- Non-ventilatory events substantially contribute to rise in pulmonary arterial blood flow with early or delayed cord clamping at birth in preterm lambs.The Journal of physiology · 2025Article
- Mild hyperbaric oxygen exposure protects heart during ischemia/reperfusion and affects vascular relaxation.Pflugers Archiv : European journal of physiology · 2024Article
- Enhanced NO-dependent pulmonary vasodilation limits increased vasoconstrictor sensitivity in neonatal chronic hypoxia.American journal of physiology. Heart and circulatory physiology · 2017Article
- The role of genetic polymorphisms in antioxidant enzymes and potential antioxidant therapies in neonatal lung disease.Antioxidants & redox signaling · 2014Review
- Sex differences in the pulmonary circulation: implications for pulmonary hypertension.American journal of physiology. Heart and circulatory physiology · 2014Review
- Inducible HSP70 regulates superoxide dismutase-2 and mitochondrial oxidative stress in the endothelial cells from developing lungs.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Fetal pulmonary arterial vascular impedance reflects changes in fetal oxygenation at near-term gestation in a nonhuman primate model.Reproductive sciences (Thousand Oaks, Calif.) · 2013Article
- Decreases in manganese superoxide dismutase expression and activity contribute to oxidative stress in persistent pulmonary hypertension of the newborn.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Mitochondrial oxidant stress increases PDE5 activity in persistent pulmonary hypertension of the newborn.Respiratory physiology & neurobiology · 2010Article
- SOD and inhaled nitric oxide normalize phosphodiesterase 5 expression and activity in neonatal lambs with persistent pulmonary hypertension.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- Shear stress stimulates nitric oxide signaling in pulmonary arterial endothelial cells via a reduction in catalase activity: role of protein kinase C delta.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- Endothelin-1 impairs nitric oxide signaling in endothelial cells through a protein kinase Cdelta-dependent activation of STAT3 and decreased endothelial nitric oxide synthase expression.DNA and cell biology · 2009Article
- Superoxide dismutase restores eNOS expression and function in resistance pulmonary arteries from neonatal lambs with persistent pulmonary hypertension.American journal of physiology. Lung cellular and molecular physiology · 2008Article
- Characterization of endothelin receptors in the peripheral lung tissues of horses unaffected and affected with recurrent airway obstruction.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2008Article
- Regulation of fibroblast growth factor-2 expression in pulmonary arterial smooth muscle cells involves increased reactive oxygen species generation.American journal of physiology. Cell physiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
At birth, ventilation and oxygenation immediately decrease pulmonary vascular resistance (PVR) and increase pulmonary blood flow (PBF); more gradual changes occur over the next several hours. Nitric oxide, produced by endothelial nitric oxide synthase (eNOS), mediates these gradual changes. To determine how ventilation and oxygenation affect eNOS gene expression, 12 fetal lambs were ventilated for 8 h without changing fetal descending aortic blood gases or pH (rhythmic distension) or with 100% oxygen (O2 ventilation). Vascular pressures and PBF were measured. Total RNA, protein, and tissue sections were prepared from lung tissue for RNase protection assays, Western blotting, and in situ hybridization. O2 ventilation increased PBF and decreased PVR more than rhythmic distension (P < 0.05). Rhythmic distension increased eNOS mRNA expression; O2 ventilation increased eNOS mRNA expression more and increased eNOS protein expression (P < 0.05). To define the mechanisms responsible for these changes, ovine fetal pulmonary arterial endothelial cells were exposed to 1, 21, or 95% O2 or to shear stress. 95% O2 increased eNOS mRNA and protein expression (P < 0.05). Shear stress increased eNOS mRNA and protein expression (P < 0.05). Increased oxygenation but more importantly increased PBF with increased shear stress induce eNOS gene expression and contribute to pulmonary vasodilation after birth.
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