ArticleApplied and environmental microbiology1997
An in vitro method for detecting infectious Cryptosporidium oocysts with cell culture.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed.
- The Synergistic Effect of Tanabesol and Calcium Hydroxide onInternational journal of veterinary science and medicine · 2026Article
- Apical-out bovine intestinal organoids as an infection model forCurrent research in parasitology & vector-borne diseases · 2025Article
- A Simple Alcohol-based Method of Oocyst Inactivation for Use in the Development of Detection Assays forFood and waterborne parasitology · 2022Article
- Microbial proteasomes as drug targets.PLoS pathogens · 2021Review
- Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture.Parasitology research · 2021Article
- Anti-protozoal activity of extracts from chicory (Cichorium intybus) against Cryptosporidium parvum in cell culture.Scientific reports · 2019Article
- Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR.Parasites & vectors · 2019Article
- Past and future trends of Cryptosporidium in vitro research.Experimental parasitology · 2019Review
- Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods.Parasite (Paris, France) · 2018Review
- In vitro inhibitory effects of plant-derived by-products against Cryptosporidium parvum.Parasite (Paris, France) · 2016Article
- CDPKs of Cryptosporidium parvum--stage-specific expression in vitro.Parasitology research · 2014Article
- Alternative indicators for monitoring Cryptosporidium oocysts in reclaimed water.Environmental science and pollution research international · 2013Article
- Inactivation of exogenous endoparasite stages by chemical disinfectants: current state and perspectives.Parasitology research · 2013Review
- Depletion of Cryptosporidium parvum oocysts from contaminated sewage by using freshwater benthic pearl clams (Hyriopsis schlegeli).Applied and environmental microbiology · 2012Article
- In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds.Parasitology research · 2012Article
- Biofilms reduce solar disinfection of Cryptosporidium parvum oocysts.Applied and environmental microbiology · 2012Article
- Assessing the effects of tertiary treated wastewater reuse on a Mediterranean river (Llobregat, NE Spain): pathogens and indicators [corrected].Environmental science and pollution research international · 2012Article
- Detection of viable Cryptosporidium parvum in soil by reverse transcription-real-time PCR targeting hsp70 mRNA.Applied and environmental microbiology · 2011Article
- Stress-induced Hsp70 gene expression and inactivation of Cryptosporidium parvum oocysts by chlorine-based oxidants.Applied and environmental microbiology · 2010Article
- Improved risk analysis by dual direct detection of total and infectious Cryptosporidium oocysts on cell culture in combination with immunofluorescence assay.Applied and environmental microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current assay methods to detect Cryptosporidium oocysts in water are generally not able to evaluate viability or infectivity. A method was developed for low-level detection of infective oocysts by using HCT-8 cells in culture as hosts to C. parvum reproductive stages. The infective foci were detected by labeling intracellular developmental stages of the parasite in an indirect-antibody assay with a primary antibody specific for reproductive stages and a secondary fluorescein isothiocyanate-conjugated antibody. The complete assay was named the focus detection method (FDM). The infectious foci (indicating that at least one of the four sporozoites released from a viable oocyst had infected a cell) were enumerated by epifluorescence microscopy and confirmed under Nomarski differential interference contrast microscopy. Time series experiments demonstrated that the autoreinfective life cycle in host HCT-8 cells began after 12 h of incubation. Through dilution studies, levels as low as one infectious oocyst were detected. The cell culture FDM compared well to other viability assays. Vital stains and excystation demonstrated that oocyst populations less than 1% viable (by vital dyes) and having a low sporozoite yield following excystation could not infect host cells. Until now, the water industry has relied on an oocyst detection method (under an information collection regulation) that is unable to determine viability. The quantifiable results of the cell culture method described demonstrate two important applications: (i) an infectivity assay that may be used in conjunction with current U.S. Environmental Protection Agency-mandated detection methodologies, and (ii) a method to evaluate oocyst infectivity in survival and disinfection studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.