ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology1997
Cytogenetics of the genus Leporinus (Pisces, Anostomidae). II. Molecular cytogenetics, organization and evolutionary conservation of a chromosome-specific satellite DNA from Leporinus obtusidens.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 20 citations in OpenAlex.
- High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax.Scientific reports · 2017Article
- Chromosome mapping of retrotransposable elementsMobile genetic elements · 2013Article
- Chromosome painting of Z and W sex chromosomes in Characidium (Characiformes, Crenuchidae).Genetica · 2013Article
- Chromosome mapping of repetitive sequences in Anostomidae species: implications for genomic and sex chromosome evolution.Molecular cytogenetics · 2012Article
- Identification of two new repetitive elements and chromosomal mapping of repetitive DNA sequences in the fish Gymnothorax unicolor (Anguilliformes: Muraenidae).European journal of histochemistry : EJH · 2011Article
- Classical and molecular cytogenetics of the zebrafish, Danio rerio (Cyprinidae, Cypriniformes): an overview.Genetica · 2001Review
- Molecular cytogenetic analysis of heterochromatin in the chromosomes of tilapia, Oreochromis niloticus (Teleostei: Cichlidae).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1998Article
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A chromosome-specific satellite DNA from the South American fish species Leporinus obtusidens has been isolated and characterized. Sequence analysis and Southern hybridization studies indicate that the cloned 483-bp fragment is 60% AT rich and appears to comprise two diverged monomers. A highly variable low-copy number polymorphism was detected and, thus, this satellite DNA may serve as a valuable genetic marker. Using a Southern blot approach, the cloned satellite DNA cross-hybridized strongly to the DNA of Leporinus elongatus but failed to detect homologous sequences in the genomes of other closely related Leporinus species and higher vertebrates. Using fluorescence in situ hybridization to mitotic metaphase spreads of L. obtusidens and L. elongatus, this satellite DNA was located to the (peri)centromeric region of one single chromosome pair in both species. As the cloned satellite DNA sequence clearly evolved along a chromosomal lineage and is highly variable, it may serve as a very useful marker in further genetic, molecular and cytogenetic studies of the genus Leporinus.
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