ArticleThe Biochemical journal1997
Induction of fatty acid synthase and S14 gene expression by glucose, xylitol and dihydroxyacetone in cultured rat hepatocytes is closely correlated with glucose 6-phosphate concentrations.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Physiological and pathological roles of lipogenesis.Nature metabolism · 2023Review
- Astaxanthin attenuates hepatic steatosis in high-fat diet-fed rats by suppressing microRNA-21 via transactivation of nuclear factor erythroid 2-related factor 2.Journal of physiology and biochemistry · 2022Article
- Proliferating tumor cells mimick glucose metabolism of mature human erythrocytes.Cell cycle (Georgetown, Tex.) · 2019Review
- ChREBP Regulates Itself and Metabolic Genes Implicated in Lipid Accumulation in β-Cell Line.PloS one · 2016Article
- Effects of xylitol on metabolic parameters and visceral fat accumulation.Journal of clinical biochemistry and nutrition · 2011Article
- Mechanisms and methods in glucose metabolism and cell death.Methods in enzymology · 2008Article
- Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.The Journal of clinical investigation · 2005Article
- Maintaining cholesterol homeostasis: sterol regulatory element-binding proteins.World journal of gastroenterology · 2004Review
- New perspectives in the regulation of hepatic glycolytic and lipogenic genes by insulin and glucose: a role for the transcription factor sterol regulatory element binding protein-1c.The Biochemical journal · 2002Review
- Characterization of the role of AMP-activated protein kinase in the regulation of glucose-activated gene expression using constitutively active and dominant negative forms of the kinase.Molecular and cellular biology · 2000Article
- Insulin stimulates pancreatic-duodenal homoeobox factor-1 (PDX1) DNA-binding activity and insulin promoter activity in pancreatic beta cells.The Biochemical journal · 1999Article
- Polyunsaturated fatty acids inhibit fatty acid synthase and spot-14-protein gene expression in cultured rat hepatocytes by a peroxidative mechanism.The Biochemical journal · 1999Article
- ADD1/SREBP-1c is required in the activation of hepatic lipogenic gene expression by glucose.Molecular and cellular biology · 1999Article
- Transcriptional regulation of the human asparagine synthetase gene by carbohydrate availability.The Biochemical journal · 1999Article
- Nutrient and hormonal regulation of pyruvate kinase gene expression.The Biochemical journal · 1999Review
- Regulation of mammalian pyruvate dehydrogenase alpha subunit gene expression by glucose in HepG2 cells.The Biochemical journal · 1998Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is now well established that the transcription of several genes belonging to the glycolytic and lipogenic pathway is stimulated in the presence of a high glucose concentration in adipocytes and hepatocytes. We have previously proposed that glucose 6-phosphate could be the signal metabolite that transduces the glucose effect. This proposal has recently been challenged and both an intermediate of the pentose phosphate pathway, xylulose 5-phosphate, and metabolites of the later part of glycolysis (3-phosphoglycerate and phosphoenolpyruvate) have been proposed. To discriminate between these possibilities, we have measured concomitantly, in primary cultures of adult rat hepatocytes, the expression of the fatty acid synthase (FAS) and S14 genes and the concentration of glucose metabolites. We have used various substrates entering at different steps of the glycolytic pathway (glucose, dihydroxyacetone) and the pentose phosphate pathway (xylitol). When compared with 5 mM glucose, 25 mM glucose induces a marked increase in both S14 and FAS gene expression, detectable as early as 2 h and peaking at 6 h. Increasing concentrations (1-5 mM) of xylitol and dihydroxyacetone in the presence of 5 mM glucose are also able to induce S14 and FAS gene expression progressively. Among the various glucose metabolites measured, glucose 6-phosphate, in contrast with xylulose 5-phosphate and metabolites of the lower part of glycolysis, is the only one that shows a clear-cut parallelism between its concentration and the degree of S14 and FAS gene expression. We conclude that glucose 6-phosphate is the most likely signal metabolite for the glucose-induced transcription of this group of genes.
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