ArticlePlant molecular biology1997
Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation.
Article in Plant molecular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Rational design of T-DNA vectors enables predictable, single-copy integration inbioRxiv : the preprint server for biology · 2026Article
- Characterization of T-Circles and Their Formation Reveal Similarities toFrontiers in plant science · 2022Article
- Studies on Pure MlbFrontiers in plant science · 2022Article
- Datura genome reveals duplications of psychoactive alkaloid biosynthetic genes and high mutation rate following tissue culture.BMC genomics · 2021Article
- Agrobacterium-mediated vacuum infiltration and floral dip transformation of rapid-cycling Brassica rapa.BMC plant biology · 2019Article
- Coexpression of octopine and succinamopine Agrobacterium virulence genes to generate high quality transgenic events in maize by reducing vector backbone integration.Transgenic research · 2018Article
- An Insight into T-DNA Integration Events in Medicago sativa.International journal of molecular sciences · 2017Article
- Analysis of T-DNA/Host-Plant DNA Junction Sequences in Single-Copy Transgenic Barley Lines.Biology · 2014Article
- Spectrum of T-DNA integrations for insertional mutagenesis of Histoplasma capsulatum.Fungal biology · 2013Article
- Formation of complex extrachromosomal T-DNA structures in Agrobacterium tumefaciens-infected plants.Plant physiology · 2012Article
- Enhanced production of single copy backbone-free transgenic plants in multiple crop species using binary vectors with a pRi replication origin in Agrobacterium tumefaciens.Transgenic research · 2011Article
- Backbone-free transformation of barrel medic (Medicago truncatula) with a Medicago-derived transfer DNA.Plant cell reports · 2010Article
- Generation of backbone-free, low transgene copy plants by launching T-DNA from the Agrobacterium chromosome.Plant physiology · 2010Article
- Generation of marker-free Bt transgenic indica rice and evaluation of its yellow stem borer resistance.Journal of applied genetics · 2010Article
- Phenome analysis in plant species using loss-of-function and gain-of-function mutants.Plant & cell physiology · 2009Review
- Plant development inhibitory genes in binary vector backbone improve quality event efficiency in soybean transformation.Transgenic research · 2008Article
- Agrobacterium-mediated transformation of embryogenic cultures and plant regeneration in Vitis rotundifolia Michx. (muscadine grape).Plant cell reports · 2008Article
- Transgene integration and organization in cotton (Gossypium hirsutum L.) genome.Transgenic research · 2008Article
- T-DNA binary vectors and systems.Plant physiology · 2008Review
- Characterization of T-DNA insertion patterns in the genome of rice blast fungus Magnaporthe oryzae.Current genetics · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When Agrobacterium was used to transform Nicotiana plumbaginifolia protoplasts and Arabidopsis thaliana roots and seedlings, a large number of plants were found in which not only the T-region defined by the border repeat sequences but the entire binary vector was integrated, as determined by both PCR and Southern analysis techniques. N. plumbaginifolia protoplast co-cultivation experiments yielded 3 out of 5 transformants with collinear sequence past the left border. In Arabidopsis root transformation experiments, 33% (6/18) of the transformants had T-DNA which exceeded the left border repeat. Vacuum infiltration of Arabidopsis seedlings produced even a greater percentage of transformants with sequences outside the left border repeat (62%, 39/63). The long transfer DNA cosegregated with the T-region encoded hygromycin resistance in the T2 progeny eliminating the possibility that long transfer DNA was of extrachromosomal or Agrobacterium origin. The high frequency of long transfer after vacuum infiltration of A. thaliana needs to be considered when analyzing T-DNA tagged mutants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.