ArticleJournal of bacteriology1997
Mutational characterization of promoter regions recognized by the Salmonella dublin virulence plasmid regulatory protein SpvR.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 18 citations in OpenAlex.
- The Role of the spv Genes in Salmonella Pathogenesis.Frontiers in microbiology · 2011Article
- Binding site determinants for the LysR-type transcriptional regulator PcaQ in the legume endosymbiont Sinorhizobium meliloti.Journal of bacteriology · 2008Article
- Characterization of the spv locus in Salmonella enterica serovar Arizona.Infection and immunity · 2002Article
- Salmonella enterica serovar Typhimurium response involved in attenuation of pathogen intracellular proliferation.Infection and immunity · 2001Article
- Two-component signal transduction as a target for microbial anti-infective therapy.Antimicrobial agents and chemotherapy · 1998Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The virulence plasmid-encoded spv regulon is essential for virulence of Salmonella dublin in mice. The spvR gene product belongs to the LysR family of transcriptional regulator proteins. SpvR induces the expression of the spvABCD operon and positively regulates its own expression. DNase I protection analysis with purified SpvR fusion proteins identified SpvR binding sites within the spvA and spvR promoters (P. Grob and D. G. Guiney, J. Bacteriol. 178:1813-1820, 1996). We have used PCR mutagenesis, combined with functional selection for reduced SpvR affinity, to define the DNA elements essential for SpvR binding. For the spvR promoter fragment, a screen for reduced expression was also applied. Sequence analysis of the resulting mutant fragments reveals that the base pair changes are clustered in distinct regions. Determination of the apparent dissociation constants of SpvR for the mutant promoters showed that the spvA LysR-type motif and the upstream palindromic sequences of both promoters play an important role in SpvR recognition.
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Registered trials
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