ArticleThe Plant cell1997
Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco.
Article in The Plant cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
67 citing papers in PubMed, 215 citations in OpenAlex.
- An improved Agrobacterium-mediated transformation method for genome editing using CRISPR/Cas9 in elite indica rice (Oryza sativa L.).BMC genomics · 2026Article
- Gene Inactivation in Transgenic Plants-A Unique Model for Studying Epigenetic Regulation of Gene Expression.Plants (Basel, Switzerland) · 2026Review
- Molecular and cytogenetic characterization of Osteospermum fruticosum lines harboring wild type pRi rol genes.PloS one · 2024Article
- CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.Frontiers in plant science · 2023Review
- Development of the binary vector pTACAtg1 for stable gene expression in plant: Reduction of gene silencing in transgenic plants carrying the target gene with long flanking sequences.Plant biotechnology (Tokyo, Japan) · 2021Article
- Article
- The M4 insulator, the TM2 matrix attachment region, and the double copy of the heavy chain gene contribute to the enhanced accumulation of the PHB-01 antibody in tobacco plants.Transgenic research · 2020Article
- Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane.Plant molecular biology · 2019Article
- CRISPR/Cas9-mediated targeted T-DNA integration in rice.Plant molecular biology · 2019Article
- Uniform Expression and Relatively Small Position Effects Characterize Sister Transformants in Maize and Soybean.Frontiers in plant science · 2019Article
- Transgenic creeping bentgrass plants expressing a Picea wilsonii dehydrin gene (PicW) demonstrate improved freezing tolerance.Molecular biology reports · 2018Article
- Coexpression of octopine and succinamopine Agrobacterium virulence genes to generate high quality transgenic events in maize by reducing vector backbone integration.Transgenic research · 2018Article
- Identification of Leaf Promoters for Use in Transgenic Wheat.Plants (Basel, Switzerland) · 2018Article
- Antifungal genes expressed in transgenic pea (Pisum sativum L.) do not affect root colonization of arbuscular mycorrhizae fungi.Mycorrhiza · 2017Article
- Isolation, phenotypic characterization and genome wide analysis of aBiotechnology for biofuels · 2017Article
- Transgenic trait deployment using designed nucleases.Plant biotechnology journal · 2016Review
- The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat.Transgenic research · 2015Article
- Effect of antibiotics on callus regeneration during transformation of IR 64 rice.Biotechnology reports (Amsterdam, Netherlands) · 2015Article
- Development of an image-based screening system for inhibitors of the plastidial MEP pathway and of protein geranylgeranylation.F1000Research · 2015Article
- Development of an optimized tetracycline-inducible expression system to increase the accumulation of interleukin-10 in tobacco BY-2 suspension cells.BMC biotechnology · 2012Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the influence of genomic context on transgene expression, we have determined the T-DNA structure, flanking DNA sequences, and chromosomal location of four independent transgene loci in tobacco. Two of these loci were stably expressed in the homozygous condition over many generations, whereas the other two loci became unstable after several generations of homozygosity. The stably expressed loci comprised relatively simple T-DNA arrangements that were flanked on at least one side by plant DNA containing AT-rich regions that bind to nuclear matrices in vitro. Of the unstably expressed loci, one consisted of multiple incomplete T-DNA copies, and the second contained a single intact T-DNA; in both cases, however, binary vector sequences were directly contiguous to a right T-DNA border. Fluorescence in situ hybridization demonstrated that the two stably expressed inserts were present in the vicinity of telomeres. The two unstably expressed inserts occupied intercalary and paracentromeric locations, respectively. Results on the stability of transgene expression in F1 progeny obtained by intercrossing the four lines and the sensitivity of the four transgene loci to inactivation in the presence of an unlinked "trans-silencing" locus are also presented. The findings are discussed in the context of repetitive DNA sequences and the allotetraploid nature of the tobacco genome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.