ArticleMolecular biology of the cell1997
The carboxyl-terminal valine residues of proTGF alpha are required for its efficient maturation and intracellular routing.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 76 citations in OpenAlex.
- Low-affinity ligands of the epidermal growth factor receptor are long-range signal transmitters in collective cell migration of epithelial cells.Cell reports · 2024Article
- Article
- The protein secretion modulator TMED9 drives CNIH4/TGFα/GLI signaling opposing TMED3-WNT-TCF to promote colon cancer metastases.Oncogene · 2019Article
- Distinct stages in the recognition, sorting, and packaging of proTGFα into COPII-coated transport vesicles.Molecular biology of the cell · 2016Article
- Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGFα.Scientific reports · 2016Article
- A biosensor for the activity of the "sheddase" TACE (ADAM17) reveals novel and cell type-specific mechanisms of TACE activation.Science signaling · 2015Article
- From wavy hair to naked proteins: the role of transforming growth factor alpha in health and disease.Seminars in cell & developmental biology · 2014Review
- Age-related neuronal loss in the cochlea is not delayed by synaptic modulation.Neurobiology of aging · 2011Article
- Testing reported associations of genetic risk factors for oral clefts in a large Irish study population.Birth defects research. Part A, Clinical and molecular teratology · 2010Article
- Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells.Molecular biology of the cell · 2007Article
- The extracellular linker of pro-neuregulin-alpha2c is required for efficient sorting and juxtacrine function.Molecular biology of the cell · 2007Article
- Substrate selectivity of epidermal growth factor-receptor ligand sheddases and their regulation by phorbol esters and calcium influx.Molecular biology of the cell · 2007Article
- Role of conserved intracellular motifs in Serrate signalling, cis-inhibition and endocytosis.The EMBO journal · 2006Article
- Dual loss of ER export and endocytic signals with altered melanosome morphology in the silver mutation of Pmel17.Molecular biology of the cell · 2006Article
- N-terminal cleavage of proTGFalpha occurs at the cell surface by a TACE-independent activity.The Biochemical journal · 2005Article
- The membrane-anchoring domain of epidermal growth factor receptor ligands dictates their ability to operate in juxtacrine mode.Molecular biology of the cell · 2005Article
- The C-terminus of prenylin is important in forming a dimer conformation necessary for endoplasmic-reticulum-to-Golgi transport.The Biochemical journal · 2004Article
- Myristoylated Naked2 escorts transforming growth factor alpha to the basolateral plasma membrane of polarized epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Transmembrane transforming growth factor-alpha tethers to the PDZ domain-containing, Golgi membrane-associated protein p59/GRASP55.The EMBO journal · 2000Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Soluble forms of transforming growth factor-alpha (TGF alpha) are derived by proteolytic processing of an integral membrane glycoprotein precursor (pro TGF alpha). Previous studies indicated that phorbol ester-induced cleavage of pro TGF alpha in CHO cells is dependent on the presence of a valine residue located at the carboxyl terminus of the precursor's cytoplasmic domain. We reassessed this requirement with epitope-tagged constructs introduced into transformed rat liver epithelial cells that normally express and process TGF alpha. We found that pro TGF alpha mutants lacking the terminal valine residues showed greatly reduced maturation to the fully glycosylated form. Additionally, they were present at substantially reduced levels on the cell surface and, instead, accumulated in the endoplasmic reticulum. Consistent with these results, enzyme-linked immunosorbent assay (ELISA) and Western blot analyses revealed little or no soluble TGF alpha in medium conditioned by cells expressing the mutant constructs. Finally, a truncated pro TGF alpha mutant lacking most of the cytoplasmic domain but retaining a carboxyl-terminal valine was processed and cleaved in a near-normal manner. These results, some of which were reproduced in CHO cells, indicate that the predominant effect of the carboxyl-terminal valines is to ensure normal maturation and routing of the precursor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.