ArticleMolecular biology of the cell1997
Mcm2 and Mcm3 are constitutive nuclear proteins that exhibit distinct isoforms and bind chromatin during specific cell cycle stages of Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- Sequence specificity of an essential nuclear localization sequence in Mcm3.PLoS genetics · 2025Article
- Sequence specificity of an essential nuclear localization sequence in Mcm3.bioRxiv : the preprint server for biology · 2024Article
- Quantity and quality of minichromosome maintenance protein complexes couple replication licensing to genome integrity.Communications biology · 2024Review
- Regulation of CLB6 expression by the cytoplasmic deadenylase Ccr4 through its coding and 3' UTR regions.PloS one · 2022Article
- Coordinated changes in cellular behavior ensure the lifelong maintenance of the hippocampal stem cell population.Cell stem cell · 2021Article
- Article
- Multiomics resolution of molecular events during a day in the life of Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Simvastatin suppresses the DNA replication licensing factor MCM7 and inhibits the growth of tamoxifen-resistant breast cancer cells.Scientific reports · 2017Article
- Cancer-derived mutations in KEAP1 impair NRF2 degradation but not ubiquitination.Cancer research · 2014Article
- Quantitative proteomic analysis of chromatin reveals that Ctf18 acts in the DNA replication checkpoint.Molecular & cellular proteomics : MCP · 2011Article
- Incremental genetic perturbations to MCM2-7 expression and subcellular distribution reveal exquisite sensitivity of mice to DNA replication stress.PLoS genetics · 2010Article
- Incorporation into the prereplicative complex activates the Mcm2-7 helicase for Cdc7-Dbf4 phosphorylation.Genes & development · 2009Article
- Nascent transcription of MCM2-7 is important for nuclear localization of the minichromosome maintenance complex in G1.Molecular biology of the cell · 2007Article
- Involvement of human MCM8 in prereplication complex assembly by recruiting hcdc6 to chromatin.Molecular and cellular biology · 2005Article
- Easy detection of chromatin binding proteins by the Histone Association Assay.Biological procedures online · 2005Article
- The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription.Nucleic acids research · 2005Article
- Eukaryotic MCM proteins: beyond replication initiation.Microbiology and molecular biology reviews : MMBR · 2004Review
- Association between histology grade, expression of HsMCM2, and cyclin A in human invasive breast carcinomas.Journal of clinical pathology · 2003Article
- Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription.Genetics · 2001Article
- Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins.Genes & development · 2000Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The Mcm2-7 proteins are a family of conserved proteins whose functions are essential for the initiation of DNA synthesis in all eukaryotes. These patients are constitutively present in high abundance in actively proliferating cells. In Saccharomyces cerevisiae, the intracellular concentrations of Mcms are between 100 and 500 times the number of replication origins. However, these proteins are limiting for the initiation of DNA synthesis at replication origins. Our studies indicate that only a small fraction of Mcm2 and Mcm3 tightly associates with chromatin, from late M phase to the beginning of the S phase. The rest of the Mcm2 and Mcm3 proteins are disturbed to both the cytoplasm and nucleoplasm in relatively constant levels throughout the cell cycle. We also show that S. cerevisiae Mcm3 is a phosphoprotein that exists in multiple isoforms and that distinct isoforms of Mcm2 and Mcm3 can be detected at specific stages of the cell cycle. These results suggest that the localization and function of the Mcm proteins are regulated by posttranslational phosphorylation in a manner that is consistent with a role for the Mcm proteins in restricting DNA replication to once per cell cycle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.