ArticleMolecular biology of the cell1997
Complexes between nascent polypeptides and their molecular chaperones in the cytosol of mammalian cells.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 101 citations in OpenAlex.
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- Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate.eLife · 2024Article
- p62 and NBR1 functions are dispensable for aggrephagy in mouse ESCs and ESC-derived neurons.Life science alliance · 2023Article
- Dual Leucine Zipper Kinase Regulates Dscam Expression through a Noncanonical Function of the Cytoplasmic Poly(A)-Binding Protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway.The Journal of cell biology · 2022Article
- Role of Hsp90 in Plasmodium falciparum Malaria.Advances in experimental medicine and biology · 2021Article
- General Structural and Functional Features of Molecular Chaperones.Advances in experimental medicine and biology · 2021Article
- Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.Molecular cell · 2020Article
- Ribopuromycylation in Coronavirus-Infected Cells.Methods in molecular biology (Clifton, N.J.) · 2020Article
- The science of puromycin: From studies of ribosome function to applications in biotechnology.Computational and structural biotechnology journal · 2020Review
- The MTM1-UBQLN2-HSP complex mediates degradation of misfolded intermediate filaments in skeletal muscle.Nature cell biology · 2018Article
- The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level.Bio-protocol · 2018Article
- A Plant Biologist's Toolbox to Study Translation.Frontiers in plant science · 2018Review
- Exploring ribosome composition and newly synthesized proteins through proteomics and potential biomedical applications.Expert review of proteomics · 2017Review
- Protein Translation Activity: A New Measure of Host Immune Cell Activation.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Article
- Conserved C-terminal nascent peptide binding domain of HYPK facilitates its chaperone-like activity.Journal of biosciences · 2014Article
- Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale.Journal of visualized experiments : JoVE · 2014Article
- Investigating the Chaperone Properties of a Novel Heat Shock Protein, Hsp70.c, from Trypanosoma brucei.Journal of parasitology research · 2014Article
- Co-translational mechanisms of quality control of newly synthesized polypeptides.Translation (Austin, Tex.) · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Folding of newly synthesized proteins in vivo is believed to be facilitated by the cooperative interaction of a defined group of proteins known as molecular chaperones. We investigated the direct interaction of chaperones with nascent polypeptides in the cytosol of mammalian cells by multiple methods. A new approach using a polyclonal antibody to puromycin allowed us to tag and capture a population of truncated nascent polypeptides with no bias as to the identity of the bound chaperones. In addition, antibodies that recognize the cytosolic chaperones hsp70, CCT (TRiC), hsp40, p48 (Hip), and hsp90 were compared on the basis of their ability to coprecipitate nascent polypeptides, both before and after chemical cross-linking. By all three approaches, hsp70 was found to be the predominant chaperone bound to nascent polypeptides. The interaction between hsp70 and nascent polypeptides is apparently dynamic under physiological conditions but can be stabilized by depletion of ATP or by cross-linking. The cytosolic chaperonin CCT was found to bind primarily to full-length, newly synthesized actin, and tubulin. We demonstrate and caution that nascent polypeptides have a propensity for binding many proteins nonspecifically in cell lysates. Although current models of protein folding in vivo have described additional components in contact with nascent polypeptides, our data indicate that the hsp70 and, perhaps, the hsp90 families are the predominant classes of molecular chaperones that interact with the general population of cytosolic nascent polypeptides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.