ArticleBiophysical journal1997
Near-simultaneous hemoglobin saturation and oxygen tension maps in mouse brain using an AOTF microscope.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 106 citations in OpenAlex.
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- Biomedical Applications of Translational Optical Imaging: From Molecules to Humans.Molecules (Basel, Switzerland) · 2021Review
- Investigation of the quantification of hemoglobin and cytochrome-c-oxidase in the exposed cortex with near-infrared hyperspectral imaging: a simulation study.Journal of biomedical optics · 2020Article
- Hyperspectral imaging solutions for brain tissue metabolic and hemodynamic monitoring: past, current and future developments.Journal of optics (2010) · 2018Review
- Noninvasive detection of acute cerebral hypoxia and subsequent matrix-metalloproteinase activity in a mouse model of cerebral ischemia using multispectral-optoacoustic-tomography.Neurophotonics · 2018Article
- ROS-responsive microspheres for on demand antioxidant therapy in a model of diabetic peripheral arterial disease.Biomaterials · 2015Article
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- In vivo hyperspectral imaging of microvessel response to trastuzumab treatment in breast cancer xenografts.Biomedical optics express · 2014Article
- In vivo spectral and fluorescence microscopy comparison of microvascular function after treatment with OXi4503, Sunitinib and their combination in Caki-2 tumors.Biomedical optics express · 2014Article
- A fast regularized least-squares method for retinal vascular oxygen tension estimation using a phosphorescence lifetime imaging model.Biomedical engineering online · 2013Article
- Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease.American journal of physiology. Heart and circulatory physiology · 2013Article
- Longitudinal in vivo imaging to assess blood flow and oxygenation in implantable engineered tissues.Tissue engineering. Part C, Methods · 2012Article
- Laser speckle contrast imaging of cerebral blood flow.Annals of biomedical engineering · 2012Review
- Wide-field functional imaging of blood flow and hemoglobin oxygen saturation in the rodent dorsal window chamber.Microvascular research · 2011Review
- In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure.Optics letters · 2011Article
- Molecular imaging of hypoxia.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2011Review
- Non-invasive, Contrast-enhanced Spectral Imaging of Breast Cancer Signatures in Preclinical Animal Models In vivo.Journal of cell science & therapy · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A newly developed microscope using acousto-optic tunable filters (AOTFs) was used to generate in vivo hemoglobin saturation (SO2) and oxygen tension (PO2) maps in the cerebral cortex of mice. SO2 maps were generated from the spectral analysis of reflected absorbance images collected at different wavelengths, and PO2 maps were generated from the phosphorescence lifetimes of an injected palladium-porphyrin compound using a frequency-domain measurement. As the inspiratory O2 was stepped from hypoxia (10% O2), through normoxia (21% O2), to hyperoxia (60% O2), measured SO2 and PO2 levels rose accordingly and predictably throughout. A plot of SO2 versus PO2 in different arterial and venous regions of the pial vessels conformed to the sigmoidal shape of the oxygen-hemoglobin dissociation curve, providing further validation of the two mapping procedures. The study demonstrates the versatility of the AOTF microscope for in vivo physiologic investigation, allowing for the generation of nearly simultaneous SO2 and PO2 maps in the cerebral cortex, and the frequency-domain detection of phosphorescence lifetimes. This class of study opens up exciting new possibilities for investigating the dynamics of hemoglobin and O2 binding during functional activation of neuronal tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.