ArticleBiophysical journal1997
AOTF microscope for imaging with increased speed and spectral versatility.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 93 citations in OpenAlex.
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- Deep-learning-assisted Fourier transform imaging spectroscopy for hyperspectral fluorescence imaging.Scientific reports · 2022Article
- Single wavelength colour tuning of spiropyran and dithienylethene based photochromic coatings.Materials advances · 2022Article
- Biomedical Applications of Translational Optical Imaging: From Molecules to Humans.Molecules (Basel, Switzerland) · 2021Review
- Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing.Light, science & applications · 2021Article
- A theoretical-experimental methodology for assessing the sensitivity of biomedical spectral imaging platforms, assays, and analysis methods.Journal of biophotonics · 2018Review
- Accuracy of full-arch scans using intraoral scanners.Clinical oral investigations · 2014Article
- Excitation-scanning hyperspectral imaging microscope.Journal of biomedical optics · 2014Article
- Thin-film tunable filters for hyperspectral fluorescence microscopy.Journal of biomedical optics · 2014Article
- Multimodal spectral imaging of cells using a transmission diffraction grating on a light microscope.Applied spectroscopy · 2011Article
- Identification of amyloid plaques in retinas from Alzheimer's patients and noninvasive in vivo optical imaging of retinal plaques in a mouse model.NeuroImage · 2011Article
- Non-invasive, Contrast-enhanced Spectral Imaging of Breast Cancer Signatures in Preclinical Animal Models In vivo.Journal of cell science & therapy · 2010Article
- Article
- Practical three color live cell imaging by widefield microscopy.Biological procedures online · 2006Article
- Spatial Distribution of Calcium Entry Evoked by Single Action Potentials within the Presynaptic Active Zone.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Computed tomography-based spectral imaging for fluorescence microscopy.Biophysical journal · 2001Article
- Macroscopic spectral imaging and gene expression analysis of the early stages of melanoma.Molecular medicine (Cambridge, Mass.) · 1999Article
- Near-simultaneous hemoglobin saturation and oxygen tension maps in mouse brain using an AOTF microscope.Biophysical journal · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a new fluorescence microscope that addresses the spectral and speed limitations of current light microscopy instrumentation. In the present device, interference and neutral density filters normally used for fluorescence excitation and detection are replaced by acousto-optic tunable filters (AOTFs). Improvements are described, including the use of a dispersing prism in conjunction with the imaging AOTF and an oblique-illumination excitation scheme, which together enable the AOTF microscope to produce images comparable to those obtained with conventional fluorescence instruments. The superior speed and spectral versatility of the AOTF microscope are demonstrated by a ratio image pair acquired in 3.5 ms and a micro-spectral absorbance measurement of hemoglobin through a cranial window in a living mouse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.