ArticleBiophysical journal1997
Kinetics of release of serotonin from isolated secretory granules. II. Ion exchange determines the diffusivity of serotonin.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 77 citations in OpenAlex.
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- Untapped Potential: Real-Time Measurements of Opioid Exocytosis at Single Cells.Journal of the American Chemical Society · 2023Article
- Sex Differences in Neurodevelopmental Disorders: A Key Role for the Immune System.Current topics in behavioral neurosciences · 2023Article
- Ion-Induced Volume Transition in Gels and Its Role in Biology.Gels (Basel, Switzerland) · 2021Review
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- Differential cargo mobilisation within Weibel-Palade bodies after transient fusion with the plasma membrane.PloS one · 2014Article
- Characterisation of Weibel-Palade body fusion by amperometry in endothelial cells reveals fusion pore dynamics and the effect of cholesterol on exocytosis.Journal of cell science · 2013Article
- Combining small-volume metabolomic and transcriptomic approaches for assessing brain chemistry.Analytical chemistry · 2013Article
- Serotonin of mast cell origin contributes to hippocampal function.The European journal of neuroscience · 2012Article
- Time-resolved release of calcium from an epithelial cell monolayer during mucin secretion.European biophysics journal : EBJ · 2011Article
- Exocytotic catecholamine release is not associated with cation flux through channels in the vesicle membrane but Na+ influx through the fusion pore.Nature cell biology · 2007Article
- A cell-semiconductor synapse: transistor recording of vesicle release in chromaffin cells.Biophysical journal · 2007Article
- Mucin granule intraluminal organization.American journal of respiratory cell and molecular biology · 2007Review
- Correlation between vesicle quantal size and fusion pore release in chromaffin cell exocytosis.Biophysical journal · 2005Article
- The force-driven conformations of heparin studied with single molecule force microscopy.Biophysical journal · 2003Article
- ATP-independent luminal oscillations and release of Ca2+ and H+ from mast cell secretory granules: implications for signal transduction.Biophysical journal · 2003Article
- Control of neurotransmitter release by an internal gel matrix in synaptic vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Mechanical forces impeding exocytotic surfactant release revealed by optical tweezers.Biophysical journal · 2003Article
- Mouse mast cell secretory granules can function as intracellular ionic oscillators.Biophysical journal · 2001Article
- Dynamics of fusion pores connecting membranes of different tensions.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We measured the efflux of 5-hydroxytryptamine (5-HT, serotonin) from an intact secretory granule extracted from the mast cell of the beige mouse. The efflux was measured with amperometry after rupture of the granule membrane was triggered by electroporation. We determined the diffusivity of 5-HT within the secretory granule to be 2.0 x 10(-8) cm2 s(-1) when the granule is in contact with a physiological saline and found that this diffusivity depends on the valence of the cation in the external electrolyte. There is a fivefold increase in the diffusion coefficient of 5-HT determined in CsCl (150 mM, pH 7.2) at 3.7 x 10(-8) cm2 s(-1) compared to that determined in histamine dihydrochloride (Hi, 100 mM at pH 4.5) at 0.7 x 10(-8) cm2 s(-1). We found that the rate of expansion of the granule matrix observed in physiological medium correlates with the efflux of 5-HT, and that the rate of swelling of the matrix and the efflux depend on the microviscosity within the granule matrix and not the bulk viscosity of the external solution. The low diffusivity of 5-HT (approximately 500-fold less than in the bulk), the observation that the valence of the counterion affects this diffusivity, and the relationship between the volume changes of the matrix and the efflux suggest that 5-HT is released from the granule by ion exchange. We discuss the implications of this result for exocytotic release in mast cells and propose that an ion exchange mechanism could control the rate of release in other secretory systems.
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