ArticleBiophysical journal1997
Kinetics of release of serotonin from isolated secretory granules. I. Amperometric detection of serotonin from electroporated granules.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework.ACS omega · 2021Article
- P2X Receptor-Dependent Modulation of Mast Cell and Glial Cell Activities in Neuroinflammation.Cells · 2021Review
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- Mast cells increase adult neural precursor proliferation and differentiation but this potential is not realized in vivo under physiological conditions.Scientific reports · 2017Article
- Puzzling Out Synaptic Vesicle 2 Family Members Functions.Frontiers in molecular neuroscience · 2017Review
- Striatal dopamine neurotransmission: regulation of release and uptake.Basal ganglia · 2016Article
- Supramolecular dynamics of mucus.Cold Spring Harbor perspectives in medicine · 2012Review
- Focal macromolecule delivery in neuronal tissue using simultaneous pressure ejection and local electroporation.Journal of neuroscience methods · 2009Article
- The crucial role of chromogranins in storage and exocytosis revealed using chromaffin cells from chromogranin A null mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Mucin granule intraluminal organization.American journal of respiratory cell and molecular biology · 2007Review
- The force-driven conformations of heparin studied with single molecule force microscopy.Biophysical journal · 2003Article
- Control of neurotransmitter release by an internal gel matrix in synaptic vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy.Biophysical journal · 2000Article
- Pulsed laser imaging of Ca(2+) influx in a neuroendocrine terminal.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Evaluation of the electrostatic field strength at the site of exocytosis in adrenal chromaffin cells.Biophysical journal · 1998Article
- Kinetics of release of serotonin from isolated secretory granules. II. Ion exchange determines the diffusivity of serotonin.Biophysical journal · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a method for measuring the efflux of 5-hydroxytryptamine (5-HT, serotonin) from isolated intact granules of the mast cell of the beige mouse. This method combines electroporation of the vesicle membrane with amperometric detection of 5-HT. A single secretory granule is placed between two platinum electrodes (distance approximately 100 microm) and positioned adjacent (<1 microm) to a carbon fiber microelectrode. A short (approximately 30 micros) high-intensity voltage pulse (electric field of approximately 5 kV/cm) is delivered to the electrodes to trigger the mechanical breakdown of the granule membrane, which activates the release of 5-HT. We observed concurrent swelling of the granule matrix with the oxidation of 5-HT at the carbon fiber electrode (overpotential + 650 mV). Similar to the release of secretory products during exocytosis, the oxidation current exhibits a spike-like time course with a noninstantaneous rising phase (time between onset of current and maximum flux, t(max)) with approximately 25% of the molecules released during this period. When the current reaches its maximum, the granule matrix attains its maximum swollen state. We found that the rising phase depends on the initial cross-sectional area of the granule (t(max) approximately 21r2) and reflects the time required for membrane rupture. The average t(1/2)spike of the amperometric spikes was found to be approximately 150 ms, which is 3-7 times faster than the t(1/2) measured during cellular exocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.