ArticleGenes & development1997
Drosophila P-element transposase is a novel site-specific endonuclease.
Article in Genes & development, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Tolerance thresholds underlie responses to DNA damage during germline development.Genes & development · 2024Article
- Article
- Activity of zebrafish THAP9 transposase and zebrafish P element-like transposons.bioRxiv : the preprint server for biology · 2024Article
- Mechanism, cellular functions and cancer roles of polymerase-theta-mediated DNA end joining.Nature reviews. Molecular cell biology · 2022Review
- Article
- CRISPR/Cas-based precision genome editing via microhomology-mediated end joining.Plant biotechnology journal · 2021Review
- Mechanism and regulation of P element transposition.Open biology · 2020Review
- Paternally Inherited P-Element Copy Number Affects the Magnitude of Hybrid Dysgenesis in Drosophila simulans and D. melanogaster.Genome biology and evolution · 2020Article
- Genetic mechanisms of formation of radiation-induced instability of the genome and its transgenerational effects in the descendants of chronically irradiated individuals of Drosophila melanogaster.Radiation and environmental biophysics · 2020Article
- Structure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts.Nature structural & molecular biology · 2019Article
- A Robust Transposon-Endogenizing Response from Germline Stem Cells.Developmental cell · 2018Article
- Review
- Targeted identification of TE insertions in aMobile DNA · 2017Article
- Drosophila IRBP bZIP heterodimer binds P-element DNA and affects hybrid dysgenesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- DNA Transposition at Work.Chemical reviews · 2016Review
- Reexamining the P-Element Invasion of Drosophila melanogaster Through the Lens of piRNA Silencing.Genetics · 2016Review
- P Transposable Elements in Drosophila and other Eukaryotic Organisms.Microbiology spectrum · 2015Review
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- An evaluation of the ecological relationship between Drosophila species and their parasitoid wasps as an opportunity for horizontal transposon transfer.Molecular genetics and genomics : MGG · 2015Article
1 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
We developed in vitro assays to study the first step of the P-element transposition reaction: donor DNA cleavage. We found that P-element transposase required both 5' and 3' P-element termini for efficient DNA cleavage to occur, suggesting that a synaptic complex forms prior to cleavage. Transposase made a staggered cleavage at the P-element termini that is novel for all known site-specific endonucleases: the 3' cleavage site is at the end of the P-element, whereas the 5' cleavage site is 17 bp within the P-element 31-bp inverted repeats. The P-element termini were protected from exonucleolytic degradation following the cleavage reaction, suggesting that a stable protein complex remains bound to the element termini after cleavage. These data are consistent with a cut-and-paste mechanism for P-element transposition and may explain why P elements predominantly excise imprecisely in vivo.
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