ArticleThe Journal of cell biology1997
Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
77 citing papers in PubMed, 182 citations in OpenAlex.
- Plakophilin 3 Is Involved in Basal Body Docking in Multiciliated Cells.International journal of molecular sciences · 2025Article
- Mitotic spindle membranes.Molecular biology of the cell · 2025Review
- Lagging Brain Gene Expression Patterns of Drosophila melanogaster Young Adult Males Confound Comparisons Between Sexes.Molecular neurobiology · 2025Article
- Contribution of intraflagellar transport to compartmentalization and maintenance of the photoreceptor cell.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Involvement of kinesins in skeletal dysplasia: a review.American journal of physiology. Cell physiology · 2024Review
- Regulation of ciliary homeostasis by intraflagellar transport-independent kinesins.Cell death & disease · 2024Review
- The two-step cargo recognition mechanism of heterotrimeric kinesin.EMBO reports · 2023Article
- IFT54 directly interacts with kinesin-II and IFT dynein to regulate anterograde intraflagellar transport.The EMBO journal · 2021Article
- Optimal sidestepping of intraflagellar transport kinesins regulates structure and function of sensory cilia.The EMBO journal · 2020Article
- KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling.The Journal of cell biology · 2020Article
- Review
- Insights into photoreceptor ciliogenesis revealed by animal models.Progress in retinal and eye research · 2019Review
- Analysis of the function of KIF3A and KIF3B in the spermatogenesis in Boleophthalmus pectinirostris.Fish physiology and biochemistry · 2018Article
- Kinesin-dependent mechanism for controlling triglyceride secretion from the liver.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery.The FEBS journal · 2017Review
- Kinesin-2 and kinesin-9 have atypical functions during ciliogenesis in the male gametophyte of Marsilea vestita.BMC cell biology · 2016Article
- Contribution of hedgehog signaling to the establishment of left-right asymmetry in the sea urchin.Developmental biology · 2016Article
- Intraflagellar transport is essential for mammalian spermiogenesis but is absent in mature sperm.Molecular biology of the cell · 2015Article
- Kinesin-2 KIF3AC and KIF3AB Can Drive Long-Range Transport along Microtubules.Biophysical journal · 2015Article
- Dynein and intraflagellar transport.Experimental cell research · 2015Review
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Heterotrimeric kinesin-II is a plus end- directed microtubule (MT) motor protein consisting of distinct heterodimerized motor subunits associated with an accessory subunit. To probe the intracellular transport functions of kinesin-II, we microinjected fertilized sea urchin eggs with an anti-kinesin-II monoclonal antibody, and we observed a dramatic inhibition of ciliogenesis at the blastula stage characterized by the assembly of short, paralyzed, 9+0 ciliary axonemes that lack central pair MTs. Control embryos show no such defect and form swimming blastulae with normal, motile, 9+2 cilia that contain kinesin-II as detected by Western blotting. Injection of anti-kinesin-II into one blastomere of a two-cell embryo leads to the development of chimeric blastulae covered on one side with short, paralyzed cilia, and on the other with normal, beating cilia. We observed a unimodal length distribution of short cilia on anti-kinesin-II-injected embryos corresponding to the first mode of the trimodal distribution of ciliary lengths observed for control embryos. This short mode may represent a default ciliary assembly intermediate. We hypothesize that kinesin-II functions during ciliogenesis to deliver ciliary components that are required for elongation of the assembly intermediate and for formation of stable central pair MTs. Thus, kinesin-II plays a critical role in embryonic development by supporting the maturation of nascent cilia to generate long motile organelles capable of producing the propulsive forces required for swimming and feeding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.