ArticleThe Journal of cell biology1997
Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 207 citations in OpenAlex.
- Mitotic spindle membranes.Molecular biology of the cell · 2025Review
- Wound Repair of the Cell Membrane: Lessons fromCells · 2024Review
- An Overview of Cell Membrane Perforation and Resealing Mechanisms for Localized Drug Delivery.Pharmaceutics · 2022Review
- Plasma membrane integrity: implications for health and disease.BMC biology · 2021Review
- Sarcolemma wounding activates dynamin-dependent endocytosis in striated muscle.The FEBS journal · 2021Article
- CaCells · 2020Review
- Mitochondria at the neuronal presynapse in health and disease.Nature reviews. Neuroscience · 2018Review
- Accumulation of Dense Core Vesicles in Hippocampal Synapses Following Chronic Inactivity.Frontiers in neuroanatomy · 2018Article
- Membrane Repair: Mechanisms and Pathophysiology.Physiological reviews · 2015Review
- Mechanisms of neuronal membrane sealing following mechanical trauma.Neuroscience bulletin · 2014Review
- Membrane damage-induced vesicle-vesicle fusion of dysferlin-containing vesicles in muscle cells requires microtubules and kinesin.Human molecular genetics · 2014Article
- Exocytosis of serotonin from the neuronal soma is sustained by a serotonin and calcium-dependent feedback loop.Frontiers in cellular neuroscience · 2014Article
- Short-term potentiation of membrane resealing in neighboring cells is mediated by purinergic signaling.Purinergic signalling · 2014Article
- Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels.Journal of cell science · 2013Article
- Opposing microtubule motors drive robust nuclear dynamics in developing muscle cells.Journal of cell science · 2012Article
- Cytoskeleton responses in wound repair.Cellular and molecular life sciences : CMLS · 2012Review
- Dysferlin and animal models for dysferlinopathy.Journal of toxicologic pathology · 2012Article
- Nonmuscle myosin IIA facilitates vesicle trafficking for MG53-mediated cell membrane repair.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Biophysics of active vesicle transport, an intermediate step that couples excitation and exocytosis of serotonin in the neuronal soma.PloS one · 2012Article
- Cell membrane disruption stimulates NO/PKG signaling and potentiates cell membrane repair in neighboring cells.PloS one · 2012Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinesin and myosin have been proposed to transport intracellular organelles and vesicles to the cell periphery in several cell systems. However, there has been little direct observation of the role of these motor proteins in the delivery of vesicles during regulated exocytosis in intact cells. Using a confocal microscope, we triggered local bursts of Ca2+-regulated exocytosis by wounding the cell membrane and visualized the resulting individual exocytotic events in real time. Different temporal phases of the exocytosis burst were distinguished by their sensitivities to reagents targeting different motor proteins. The function blocking antikinesin antibody SUK4 as well as the stalk-tail fragment of kinesin heavy chain specifically inhibited a slow phase, while butanedione monoxime, a myosin ATPase inhibitor, inhibited both the slow and fast phases. The blockage of Ca2+/calmodulin-dependent protein kinase II with autoinhibitory peptide also inhibited the slow and fast phases, consistent with disruption of a myosin-actin- dependent step of vesicle recruitment. Membrane resealing after wounding was also inhibited by these reagents. Our direct observations provide evidence that in intact living cells, kinesin and myosin motors may mediate two sequential transport steps that recruit vesicles to the release sites of Ca2+-regulated exocytosis, although the identity of the responsible myosin isoform is not yet known. They also indicate the existence of three semistable vesicular pools along this regulated membrane trafficking pathway. In addition, our results provide in vivo evidence for the cargo-binding function of the kinesin heavy chain tail domain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.