ArticleThe Journal of cell biology1997
Dynamics of ATP-induced calcium signaling in single mouse thymocytes.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- The P2X7 receptor in mucosal adaptive immunity.Purinergic signalling · 2024Review
- Magnesium protects against sepsis by blocking gasdermin D N-terminal-induced pyroptosis.Cell death and differentiation · 2020Article
- The P2X7 Receptor as Regulator of T Cell Development and Function.Frontiers in immunology · 2020Article
- Ion channels in innate and adaptive immunity.Annual review of immunology · 2015Review
- Purinergic signalling and immune cells.Purinergic signalling · 2014Review
- Putative roles of purinergic signaling in human immunodeficiency virus-1 infection.Biology direct · 2014Article
- Purinergic signalling in endocrine organs.Purinergic signalling · 2014Review
- Barcoding T cell calcium response diversity with methods for automated and accurate analysis of cell signals (MAAACS).PLoS computational biology · 2013Article
- Article
- Immunodeficiency due to defects in store-operated calcium entry.Annals of the New York Academy of Sciences · 2011Review
- Measuring enzyme activity in single cells.Trends in biotechnology · 2011Review
- Recent patents on novel P2X(7) receptor antagonists and their potential for reducing central nervous system inflammation.Recent patents on CNS drug discovery · 2010Review
- ORAI1 and STIM1 deficiency in human and mice: roles of store-operated Ca2+ entry in the immune system and beyond.Immunological reviews · 2009Review
- Expression of P2 receptors in human B cells and Epstein-Barr virus-transformed lymphoblastoid cell lines.BMC immunology · 2006Article
- Effect of melatonin on phagocytic activity and intracellular free calcium concentration in testicular macrophages from normal and streptozotocin-induced diabetic rats.Molecular and cellular biochemistry · 2005Article
- Microfluorimetric analysis of a purinergic receptor (P2X7) in GH4C1 rat pituitary cells: effects of a bioactive substance produced by Pfiesteria piscicida.Environmental health perspectives · 2001Article
- Molecular properties and physiological roles of ion channels in the immune system.Journal of clinical immunology · 2001Review
- Relations between intracellular Ca2+ stores and store-operated Ca2+ entry in primary cultured human glioblastoma cells.The Journal of physiology · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Extracellular ATP (ATPo) elicits a robust change in the concentration of intracellular Ca2+ ([Ca2+]i) in fura-2-loaded mouse thymocytes. Most thymocytes (60%) exposed to ATPo exhibited a biphasic rise in [Ca2+]i; [Ca2+]i rose slowly at first to a mean value of 260 nM after 163 s and then increased rapidly to a peak level of 735 nM. In many cells, a declining plateau, which lasted for more than 10 min, followed the crest in [Ca2+]i. Experiments performed in the absence of extracellular [Ca2+]o abolished the rise in thymocyte [Ca2+]i, indicating that Ca2+ influx, rather than the release of stored Ca2+, is stimulated by ATPo. ATPo- mediated Ca2+ influx was potentiated as the [Mg2+]o was reduced, confirming that ATP4- is the active agonist form. In the absence of Mg2+o, 3'-O-(4-benzoyl)benzoyl-ATP (BzATP) proved to be the most effective agonist of those tested. The rank order of potency for adenine nucleotides was BzATP4->ATP4->MgATP2->ADP3-, suggesting purinoreceptors of the P2X7/P2Z class mediate the ATPo response. Phenotyping experiments illustrate that both immature (CD4-CD8-, CD4+CD8+) and mature (CD4+CD8-, CD4-CD8+) thymocyte populations respond to ATP. Further separation of the double-positive population by size revealed that the ATPo-mediated [Ca2+]i response was much more pronounced in large (actively dividing) than in small (terminally differentiated) CD4+CD8+ thymocytes. We conclude that thymocytes vary in sensitivity to ATPo depending upon the degree of maturation and suggest that ATPo may be involved in processes that control cellular differentiation within the thymus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.