ArticleNucleic acids research1997
Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Coming Together: RNAs and Proteins Assemble under the Single-Molecule Fluorescence Microscope.Cold Spring Harbor perspectives in biology · 2019Review
- Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology.Archaea (Vancouver, B.C.) · 2015Review
- Single molecule fluorescence approaches shed light on intracellular RNAs.Chemical reviews · 2014Review
- A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence.RNA biology · 2013Article
- Structural bias in T4 RNA ligase-mediated 3'-adapter ligation.Nucleic acids research · 2012Article
- Fluorescent DNA-based enzyme sensors.Chemical Society reviews · 2011Review
- Two-step aminoacylation of tRNA without channeling in Archaea.Journal of molecular biology · 2011Article
- Isolation of translating ribosomes containing peptidyl-tRNAs for functional and structural analyses.Journal of visualized experiments : JoVE · 2011Article
- Protein analysis based on molecular beacon probes and biofunctionalized nanoparticles.Science China. Chemistry · 2010Review
- Enzymes used in molecular biology: a useful guide.Journal of cell communication and signaling · 2008Article
- An RNA toolbox for single-molecule force spectroscopy studies.Nucleic acids research · 2007Article
- Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties.Nucleic acids research · 2005Article
- Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.Nucleic acids research · 2003Article
- Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons.Nucleic acids research · 2003Article
- Oligoribonucleotide circularization by 'template-mediated' ligation with T4 RNA ligase: synthesis of circular hammerhead ribozymes.Nucleic acids research · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.