ArticleNucleic acids research1997
Oligonucleotides with conjugated dihydropyrroloindole tripeptides: base composition and backbone effects on hybridization.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- A rapid minor groove binder PCR method for distinguishing the vaccine strain Brucella abortus 104M.BMC veterinary research · 2018Article
- Rapid identification and quantification of Campylobacter coli and Campylobacter jejuni by real-time PCR in pure cultures and in complex samples.BMC microbiology · 2011Article
- Association of DDAH2 gene polymorphism with cardiovascular disease in Egyptian patients.Journal of genetics · 2011Article
- Use of base modifications in primers and amplicons to improve nucleic acids detection in the real-time snake polymerase chain reaction.Assay and drug development technologies · 2011Article
- New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Forster resonance energy transfer probes in 5'-nuclease assays.Nucleic acids research · 2010Article
- Rapid real-time PCR assays for detection of Klebsiella pneumoniae with the rmpA or magA genes associated with the hypermucoviscosity phenotype: screening of nonhuman primates.The Journal of molecular diagnostics : JMD · 2009Article
- Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis.BMC microbiology · 2008Article
- Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR.Nucleic acids research · 2007Article
- Detection and genotyping of varicella-zoster virus by TaqMan allelic discrimination real-time PCR.Journal of clinical microbiology · 2004Article
- Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse Probe System in challenging sequence environment.Human genomics · 2004Article
- Fluorescence polarization in homogeneous nucleic acid analysis II: 5'-nuclease assay.Genome research · 2001Article
- 3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures.Nucleic acids research · 2000Article
- Solution structure of a highly stable DNA duplex conjugated to a minor groove binder.Nucleic acids research · 1998Article
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4 authors.
Funding
Abstract
The ability of conjugated minor groove binding (MGB) residues to stabilize nucleic acid duplexes was investigated by synthesis of oligonucleotides bearing a tethered dihydropyrroloindole tripeptide (CDPI3). Duplexes bearing one or more of these conjugated MGBs were varied by base composition (AT- or GC-rich oligonucleotides), backbone modifications (phosphodiester DNA, 2'-O-methyl phosphodiester RNA or phosphorothioate DNA) and site of attachment of the MGB moiety (5'- or 3'-end of either duplex strand). Melting temperatures of the duplexes were determined. The conjugated CDPI3 residue enhanced the stability of virtually all duplexes studied. The extent of stabilization was backbone and sequence dependent and reached a maximum value of 40-49 degrees C for d(pT)8. d(pA)8. Duplexes with a phosphorothioate DNA backbone responded similarly on CDPI3 conjugation, although they were less stable than analogous phosphodiesters. Modest stabilization was obtained for duplexes with a 2'-O-methyl RNA backbone. The conjugated CDPI3 residue stabilized GC-rich DNA duplexes, albeit to a lesser extent than for AT-rich duplexes of the same length.
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