ArticleNucleic acids research1997
Fine mapping of replication origins (ori A and ori B) in Nicotiana tabacum chloroplast DNA.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 82 citations in OpenAlex.
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- Mini-synplastomes for plastid genetic engineering.Plant biotechnology journal · 2022Article
- The complete chloroplast genome sequencing analysis revealed an unusual IRs reduction in three species of subfamily Zygophylloideae.PloS one · 2022Article
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Lost and Found: Return of the Inverted Repeat in the Legume Clade Defined by Its Absence.Genome biology and evolution · 2019Article
- Assembly of chloroplast genomes with long- and short-read data: a comparison of approaches using Eucalyptus pauciflora as a test case.BMC genomics · 2018Article
- Efficient Replication of the Plastid Genome Requires an Organellar Thymidine Kinase.Plant physiology · 2018Article
- RNase H1 Cooperates with DNA Gyrases to Restrict R-Loops and Maintain Genome Integrity in Arabidopsis Chloroplasts.The Plant cell · 2017Article
- Degeneration of photosynthetic capacity in mixotrophic plants,Plant diversity · 2017Article
- Variable presence of the inverted repeat and plastome stability in Erodium.Annals of botany · 2016Article
- Codon Adaptation of Plastid Genes.PloS one · 2016Article
- Chloroplast DNA Copy Number Changes during Plant Development in Organelle DNA Polymerase Mutants.Frontiers in plant science · 2016Article
- A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNAIle(GAU).Plant physiology · 2015Article
- A precise chloroplast genome of Nelumbo nucifera (Nelumbonaceae) evaluated with Sanger, Illumina MiSeq, and PacBio RS II sequencing platforms: insight into the plastid evolution of basal eudicots.BMC plant biology · 2014Article
- Comparative genomics of ten solanaceous plastomes.Advances in bioinformatics · 2014Article
- The chloroplast genome of Hyoscyamus niger and a phylogenetic study of the tribe Hyoscyameae (Solanaceae).PloS one · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a partially purified replication complex from tobacco chloroplasts, replication origins have been localized to minimal sequences of 82 (pKN8, positions 137 683-137 764) and 243 bp (pKN3, positions 130 513-130 755) for ori A and ori B respectively. Analysis of in vitro replication products by two-dimensional agarose gel electrophoresis showed simple Y patterns for single ori sequence-containing clones, indicative of rolling circle replication. Double Y patterns were observed when a chloroplast DNA template containing both ori s (pKN9) was tested. Dpn I analysis and control assays with Escherichia coli DNA polymerase provide a clear method to distinguish between true replication and DNA repair synthesis. These controls also support the reliability of this in vitro chloroplast DNA replication system. EM analysis of in vitro replicated products showed rolling circle replication intermediates for single ori clones (ori A or ori B), whereas D loops were observed for a clone (pKN9) containing both ori s. The minimal ori regions contain sequences which are capable of forming stem-loop structures with relatively high free energy and other sequences which interact with specific protein(s) from the chloroplast replication fraction. Apparently the minimal ori sequences reported here contain all the necessary elements for support of chloroplast DNA replication in vitro.
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