ArticlePlant physiology1997
Expression and native structure of cytosolic class II small heat-shock proteins.
Article in Plant physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions.Science (New York, N.Y.) · 2018Article
- Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress.Plant physiology · 2016Article
- The Mechanisms of Maize Resistance toFrontiers in plant science · 2016Article
- Small heat shock protein Hsp17.8 functions as an AKR2A cofactor in the targeting of chloroplast outer membrane proteins in Arabidopsis.Plant physiology · 2011Article
- The small heat shock protein 20 RSI2 interacts with and is required for stability and function of tomato resistance protein I-2.The Plant journal : for cell and molecular biology · 2010Article
- Mechanistic differences between two conserved classes of small heat shock proteins found in the plant cytosol.The Journal of biological chemistry · 2010Article
- Functional isolation of novel nuclear proteins showing a variety of subnuclear localizations.The Plant cell · 2005Article
- Tomato heat stress protein Hsp16.1-CIII represents a member of a new class of nucleocytoplasmic small heat stress proteins in plants.Cell stress & chaperones · 2003Article
- Analysis of interactions between domains of a small heat shock protein, Hsp30 of Neurospora crassa.Cell stress & chaperones · 2002Article
- Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network.Microbiology and molecular biology reviews : MMBR · 2002Review
- The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing alpha-crystallin domains (Acd proteins).Cell stress & chaperones · 2001Article
- Chloroplast small heat shock proteins: evidence for atypical evolution of an organelle-localized protein.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Messenger RNA-binding properties of nonpolysomal ribonucleoproteins from heat-stressed tomato cellsPlant physiology · 1999Article
- The chloroplast small heat-shock protein oligomer is not phosphorylated and does not dissociate during heat stress in vivo.Plant physiology · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Higher plants synthesize small heat-shock proteins (smHSPs) from five related gene families. The class I and II families encode cytosolic smHSPs. We characterized the class II smHSPs of pea (Pisum sativum) and compared them with class I smHSPs. Antibodies against recombinant HSP17.7, a class II smHSP, recognized four heat-inducible 17- to 18-kD polypeptides and did not cross-react with class I smHSPs. On sucrose gradients the class II smHSPs sedimented primarily at 8 Svedberg units, indicating that they are components of large complexes similar in size to class I smHSP complexes. However, the class I and II complexes were readily distinguishable by nondenaturing polyacrylamide gel electrophoresis and isoelectric focusing. Nondenaturing immune precipitations using anti-HSP17.7 or anti-HSP18.1 (a class I smHSP) antiserum provide further evidence that the class I and II smHSPs exist in different complexes, composed primarily of smHSPs. Recombinant HSP17.7 and HSP18.1 formed complexes of sizes similar to those formed in vivo. When these two smHSPs were mixed, denatured with urea, and then dialyzed, the distinct class I and II complexes again formed, each containing only HSP18.1 or HSP17.7. Thus, cytosolic smHSPs from two related gene families expressed simultaneously form distinct complexes in vivo, suggesting that they have subtly different functions.
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