ArticleThe Journal of general physiology1997
Plasma membrane water permeability of cultured cells and epithelia measured by light microscopy with spatial filtering.
Article in The Journal of general physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 115 citations in OpenAlex.
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- Molecular imaging with aquaporin-based reporter genes: quantitative considerations from Monte Carlo diffusion simulations.bioRxiv : the preprint server for biology · 2023Article
- Methods to Measure Water Permeability.Advances in experimental medicine and biology · 2023Article
- Hydrostatic pressure as a driver of cell and tissue morphogenesis.Seminars in cell & developmental biology · 2022Review
- The importance of water and hydraulic pressure in cell dynamics.Journal of cell science · 2020Review
- Cell Volume Changes and Membrane Ruptures Induced by Hypotonic Electrolyte and Sugar Solutions.Frontiers in physiology · 2020Article
- A Reverse-Osmosis Model of Apoptotic Shrinkage.Frontiers in cell and developmental biology · 2020Article
- Quantitative Model for Ion Transport and Cytoplasm Conductivity of Chinese Hamster Ovary Cells.Scientific reports · 2018Article
- Refractive Index Imaging of Cells with Variable-Angle Near-Total Internal Reflection (TIR) Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2017Article
- Evaluation and comparison of diffusion MR methods for measuring apparent transcytolemmal water exchange rate constant.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2017Article
- The role of osmolality in saline fluid nebulization after tracheostomy: time for changing?BMC pulmonary medicine · 2016Article
- Osmotic Effects Induced by Pore-Forming Agent Nystatin: From Lipid Vesicles to the Cell.PloS one · 2016Article
- Detecting Aquaporin Function and Regulation.Frontiers in chemistry · 2016Review
- A spatial model of fluid recycling in the airways of the lung.Journal of theoretical biology · 2015Article
- Quantification of surface tension and internal pressure generated by single mitotic cells.Scientific reports · 2014Article
- Aquaporins: important but elusive drug targets.Nature reviews. Drug discovery · 2014Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A method was developed to measure the osmotic water permeability (Pf) of plasma membranes in cell layers and applied to cells and epithelia expressing molecular water channels. It was found that the integrated intensity of monochromatic light in a phase contrast or dark field microscope was dependent on relative cell volume. For cells of different size and shape (Sf9, MDCK, CHO, A549, tracheal epithelia, BHK), increased cell volume was associated with decreased signal intensity; generally the signal decreased 10-20% for a twofold increase in cell volume. A theory relating signal intensity to relative cell volume was developed based on spatial filtering and changes in optical path length associated with cell volume changes. Theory predictions were confirmed by signal measurements of cell layers bathed in solutions of various osmolarities and refractive indices. The excellent signal-to-noise ratio of the transmitted light detection permitted measurement of cell volume changes of <1%. The method was applied to characterize transfected cells and tissues that natively express water channels. Pf in control Chinese hamster ovary cells was low (0.0012 cm/s at 23 degrees C) and increased more than fourfold upon stable transfection with aquaporins 1, 2, 4, or 5. Pf in apical and basolateral membranes in polarized epithelial cells grown on porous supports was measured. Pfbl and Pfap were 0.0011 and 0.0024 cm/s (MDCK cells), and 0.0039 and 0.0052 cm/s (human tracheal cells) at 23 degrees C. In intact toad urinary bladder, basolateral Pf was 0.036 cm/s and apical membrane Pf after vasopressin stimulation was 0.025 cm/s at 23 degrees C. The results establish light microscopy with spatial filtering as a technically simple and quantitative method to measure water permeability in cell layers and provide the first measurement of the apical and basolateral membrane permeabilities of several important epithelial cell types.
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