ArticleThe Journal of clinical investigation1997
Heparin-binding ligands mediate autocrine epidermal growth factor receptor activation In skin organ culture.
Article in The Journal of clinical investigation, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 108 citations in OpenAlex.
- Impaired wound healing in Parkinson's disease: a hypothesis on altered epidermal growth factor (EGF) and N-methyl-D-aspartate (NMDA) signaling in keratinocytes.Molecular medicine (Cambridge, Mass.) · 2025Article
- Nuclear IL-33 Plays an Important Role in EGFR-Mediated Keratinocyte Migration by Regulating the Activation of Signal Transducer and Activator of Transcription 3 and NF-κB.JID innovations : skin science from molecules to population health · 2023Article
- Spatial activation of ezrin by epidermal growth factor receptor and focal adhesion kinase co-ordinates epithelial cell migration.Open biology · 2021Article
- The immunopathology of lung fibrosis: amphiregulin-producing pathogenic memory T helper-2 cells control the airway fibrotic responses by inducing eosinophils to secrete osteopontin.Seminars in immunopathology · 2019Review
- Piperonylic acid stimulates keratinocyte growth and survival by activating epidermal growth factor receptor (EGFR).Scientific reports · 2018Article
- Dysfunction of the circadian transcriptional factor CLOCK in mice resists chemical carcinogen-induced tumorigenesis.Scientific reports · 2017Article
- Epithelial-mesenchymal transition in tissue repair and fibrosis.Cell and tissue research · 2016Review
- Article
- Transgenic expression of human amphiregulin in mouse skin: inflammatory epidermal hyperplasia and enlarged sebaceous glands.Experimental dermatology · 2016Article
- Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.The Journal of investigative dermatology · 2016Article
- Pulmonary epithelial barrier function: some new players and mechanisms.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- EGFR signaling blunts allergen-induced IL-6 production and Th17 responses in the skin and attenuates development and relapse of atopic dermatitis.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Human epidermal growth factor receptor signaling in acute lung injury.American journal of respiratory cell and molecular biology · 2012Review
- Amphiregulin-dependent mucous cell metaplasia in a model of nonallergic lung injury.American journal of respiratory cell and molecular biology · 2012Article
- Heparin-binding EGF-like growth factor promotes epithelial-mesenchymal transition in human keratinocytes.The Journal of investigative dermatology · 2012Article
- MicroRNA-132 targets HB-EGF upon IgE-mediated activation in murine and human mast cells.Cellular and molecular life sciences : CMLS · 2012Article
- Enhanced epithelial-mesenchymal transition-like phenotype in N-acetylglucosaminyltransferase V transgenic mouse skin promotes wound healing.The Journal of biological chemistry · 2011Article
- The Roles of ADAMs Family Proteinases in Skin Diseases.Enzyme research · 2011Article
- Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells.Molecular vision · 2011Article
- A novel signaling pathway of tissue kallikrein in promoting keratinocyte migration: activation of proteinase-activated receptor 1 and epidermal growth factor receptor.Experimental cell research · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Exogenous EGF and TGF-alpha accelerate wound healing, but treatment effects are often modest. Using short-term human skin organ culture, we found that autocrine EGF receptor activation could account for this observation. Amphiregulin and heparin-binding EGF-like growth factor (HB-EGF) transcripts were rapidly and markedly induced, whereas EGF and TGF-alpha mRNAs were undetectable or only slightly increased. Vascular permeability factor and keratin 6 transcripts were also strongly induced, albeit with a >/= 3 h delay relative to HB-EGF and amphiregulin. All four transcripts were upregulated in actual healing skin wounds, HB-EGF and keratin 6 being the most prominent. The highly EGF receptor-specific tyrosine kinase inhibitor PD153035 strongly inhibited induction of all four transcripts in organ culture, as well as release of immunoreactive HB-EGF into the medium. These effects were confirmed using the anti-EGF receptor mAb 225 IgG. Neither PD153035 nor 225 IgG was toxic to keratinocytes, as judged by calcein-AM uptake. PD153035 completely abrogated the proliferative phase of keratinocyte outgrowth in skin explant cultures, whereas it had no effect on the antecedent migratory phase. Based on these results, we conclude that EGF receptor activation by highly inducible, keratinocyte-derived heparin-binding ligands is an important mechanism for amplification and transmission of the cutaneous wound healing signal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.