ArticleJournal of clinical microbiology1997
Evaluation of a commercial ligase chain reaction kit (Abbott LCx) for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary specimens.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Use of technetium(99m)-ciprofloxacin scan in Pott's spine to assess the disease activity.International orthopaedics · 2012Article
- Detection of non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors.Sensors (Basel, Switzerland) · 2010Article
- Rapid and specific detection of Mycobacterium tuberculosis by using the Smart Cycler instrument and a specific fluorogenic probe.Journal of clinical microbiology · 2003Article
- Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples: a multicenter study.Journal of clinical microbiology · 2003Article
- Tuberculous orchiepididymitis diagnosed by nucleic acid amplification test: a case report.International urology and nephrology · 2003Article
- Rapid and specific detection of Mycobacterium tuberculosis from acid-fast bacillus smear-positive respiratory specimens and BacT/ALERT MP culture bottles by using fluorogenic probes and real-time PCR.Journal of clinical microbiology · 2002Article
- Is the detection of Mycobacterium tuberculosis DNA by ligase chain reaction worth the cost: experiences from an inner London teaching hospital.Journal of clinical pathology · 2000Article
- Evaluation of the LiPA MYCOBACTERIA assay for identification of mycobacterial species from BACTEC 12B bottles.Journal of clinical microbiology · 2000Article
- Multicenter evaluation of the Abbott LCx Mycobacterium tuberculosis ligase chain reaction assay.Journal of clinical microbiology · 1999Article
- Evaluation of three nucleic acid amplification methods for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.Journal of clinical microbiology · 1999Article
- Evaluation of the Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in human samples.Journal of clinical microbiology · 1999Article
- Comparative evaluation of the new gen-probe Mycobacterium tuberculosis amplified direct test and the semiautomated abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in respiratory and extrapulmonary specimens.Journal of clinical microbiology · 1998Article
- Rapid diagnosis of pulmonary tuberculosis with the LCx Mycobacterium tuberculosis assay and comparison with conventional diagnostic techniques.Journal of clinical microbiology · 1998Article
- Early detection of Mycobacterium tuberculosis in BACTEC cultures by ligase chain reaction.Journal of clinical microbiology · 1998Article
- Rapid diagnosis of extrapulmonary tuberculosis by ligase chain reaction amplification.Journal of clinical microbiology · 1998Article
- Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by ligase chain reaction.Journal of clinical microbiology · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct detection of Mycobacterium tuberculosis by means of a commercial ligase chain reaction DNA amplification method (LCx M. tuberculosis; Abbott Diagnostics Division, Abbott Park, Ill.) was investigated with 511 (including 147 extrarespiratory) specimens collected from 358 patients. LCx results were compared with standard microbiological data, and conflicting cases were resolved according to the final clinical diagnosis. M. tuberculosis was detected in 45 of 358 subjects by means of the LCx test. The test was negative for all 30 specimens with mycobacteria other than M. tuberculosis. The sensitivity, specificity, and positive and negative predictive values for the LCx test, compared with culture results, were 93.90, 92.31, 70.00, and 98.75%, respectively; these values rose in resolved cases to 95.53, 99.25, 97.27, and 98.75%, respectively. With respiratory specimens, for which the LCx system is licensed, the sensitivity reached 98.97%. In patients with a final clinical diagnosis of tuberculosis the sensitivity of the LCx system was 89.36% compared to 82.98% for cultures and 78.72% for microscopy. We conclude that the LCx test is user friendly, rapid, fairly sensitive, and highly specific. It can also be effectively used on extrapulmonary specimens provided possible false-negative results are taken into account. However, the use of LCx test appears to be less appropriate for the monitoring of antituberculosis therapy, as the majority of samples from treated tuberculosis patients gave consistently positive results, despite the sterilization of cultures.
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