ArticleJournal of clinical microbiology1997
Culturing selects for specific genotypes of Borrelia burgdorferi in an enzootic cycle in Colorado.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- The evolving story of Borrelia burgdorferi sensu lato transmission in Europe.Parasitology research · 2022Review
- Comparison of MKP and BSK-H media for the cultivation and isolation of Borrelia burgdorferi sensu lato.PloS one · 2017Article
- Lyme borreliosis in human patients in Florida and Georgia, USA.International journal of medical sciences · 2013Article
- Genotypic variation and mixtures of Lyme Borrelia in Ixodes ticks from North America and Europe.PloS one · 2010Article
- Population structure of the lyme borreliosis spirochete Borrelia burgdorferi in the western black-legged tick (Ixodes pacificus) in Northern California.Applied and environmental microbiology · 2009Article
- Borrelia burgdorferi sensu stricto is clonal in patients with early Lyme borreliosis.Applied and environmental microbiology · 2008Article
- MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Genetic diversity of Borrelia burgdorferi sensu stricto in Peromyscus leucopus, the primary reservoir of Lyme disease in a region of endemicity in southern Maryland.Applied and environmental microbiology · 2006Article
- Serologic proteome analysis of Borrelia burgdorferi membrane-associated proteins.Infection and immunity · 2006Article
- Molecular identification and analysis of Borrelia burgdorferi sensu lato in lizards in the southeastern United States.Applied and environmental microbiology · 2005Article
- Comparison of Borrelia burgdorferi sensu lato strains isolated from specimens obtained simultaneously from two different sites of infection in individual patients.Journal of clinical microbiology · 2005Article
- Borrelia burgdorferi ospC heterogeneity among human and murine isolates from a defined region of northern Maryland and southern Pennsylvania: lack of correlation with invasive and noninvasive genotypes.Journal of clinical microbiology · 2005Article
- Variations in Barbour-Stoenner-Kelly culture medium modulate infectivity and pathogenicity of Borrelia burgdorferi clinical isolates.Infection and immunity · 2004Article
- Borrelia species in host-seeking ticks and small mammals in northern Florida.Journal of clinical microbiology · 2004Article
- Spotted-fever group Rickettsia in Dermacentor variabilis, Maryland.Emerging infectious diseases · 2004Article
- Prevalence of Borrelia burgdorferi species and identification of Borrelia valaisiana in questing Ixodes ricinus in the Lyon region of France as determined by polymerase chain reaction-restriction fragment length polymorphism.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2003Article
- Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-single-strand conformation polymorphism analysis of the rrfA-rrlB intergenic spacer in ixodes ricinus ticks from the Czech Republic.Applied and environmental microbiology · 2003Article
- Disease severity in a murine model of lyme borreliosis is associated with the genotype of the infecting Borrelia burgdorferi sensu stricto strain.The Journal of infectious diseases · 2002Article
- Article
- Genetic diversity of Borrelia burgdorferi sensu lato in ticks from mainland Portugal.Journal of clinical microbiology · 2000Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Colorado, Borrelia burgdorferi sensu stricto, the etiologic agent of Lyme disease, is maintained in an enzootic cycle between Ixodes spinipalpis ticks and Neotoma mexicana rats (27). The frequencies of flagellin (fla), 66-kDa protein (p66), and outer surface protein A (ospA) alleles were examined in 71 B. burgdorferi isolates from samples from Colorado. Approximately two-thirds of these samples were isolates from I. spinipalpis ticks that had been cultured in BSK-H medium prior to DNA extraction. The remaining samples were from total DNA extracted directly from infected I. spinipalpis ticks. A portion of each gene was amplified by PCR and screened for genetic variability by single-strand conformation polymorphism (SSCP) analysis. We identified three alleles in the fla gene, seven in the p66 gene, and seven in the ospA gene. Sequencing verified that the amplified products originated from B. burgdorferi template DNA and indicated 100% sensitivity and specificity of the SSCP analysis. The frequencies of the p66 and ospA alleles were significantly different between cultured and uncultured spirochetes. The number of three-locus genotypes and the genetic diversity of alleles at all loci were consistently lower in cultured spirochetes, suggesting that culturing of B. burgdorferi in BSK-H medium may select for specific genotypes.
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