ArticleJournal of clinical microbiology1997
Effects of storage temperature and time on qualitative and quantitative detection of cytomegalovirus in blood specimens by shell vial culture and PCR.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Safety and efficacy of prolonged cytomegalovirus prophylaxis with intravenous ganciclovir in pediatric and young adult lung transplant recipients.Pediatric transplantation · 2007Trial
- Is Neonatal Viremia a Possible Predictor of the Timing of Maternal Infection in Asymptomatic Congenital Cytomegalovirus Infection? A Retrospective Study.Journal of personalized medicine · 2025Article
- Evaluation of the Tasso+ blood self-collection device for quantitation of plasma cytomegalovirus (CMV) DNAemia in adult solid organ transplant recipients (SOTr).Microbiology spectrum · 2024Article
- Stability of severe acute respiratory syndrome coronavirus 2 RNA in placenta and fetal cells.American journal of obstetrics and gynecology · 2021Article
- Clinical utility of viral load in management of cytomegalovirus infection after solid organ transplantation.Clinical microbiology reviews · 2013Review
- Interpreting quantitative cytomegalovirus DNA testing: understanding the laboratory perspective.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- Cytomegalovirus DNA stability in EDTA anti-coagulated whole blood and plasma samples.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2011Article
- Overview of the diagnosis of cytomegalovirus infection.Infectious disorders drug targets · 2011Article
- Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients.Journal of clinical microbiology · 2003Article
- Multiplex, quantitative, real-time PCR assay for cytomegalovirus and human DNA.Journal of clinical microbiology · 2002Article
- Predictive value of quantitative PCR-based viral burden analysis for eight human herpesviruses in pediatric solid organ transplant patients.The Journal of molecular diagnostics : JMD · 2000Article
- Comparison of PCR, antigenemia assay, and rapid blood culture for detection and prevention of cytomegalovirus disease after lung transplantation.Journal of clinical microbiology · 2000Article
- New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients.Clinical microbiology reviews · 2000Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytomegalovirus (CMV) infectious titers and DNA levels were determined by quantitative shell vial culture and quantitative-competitive PCR with blood samples from 10 renal transplant recipients with active CMV infection. Blood samples were stored at either room temperature or 4 degrees C and were processed at intervals of 0, 6, 24, 48, and 72 h. All samples were culture and PCR positive at baseline. Whereas the sensitivity of shell vial culture progressively declined, with only 55% positive at 24 h and 10% positive at 48 h, all samples remained PCR positive at all time points. Furthermore, the infectious titer diminished by 83 to 91% by 24 h compared to that at baseline (P < 0.0001), but quantitative DNA levels did not decline over time. Storage temperature had no significant effect on either infectious titer or DNA levels.
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