ArticleProceedings of the National Academy of Sciences of the United States of America1997
Operons and SL2 trans-splicing exist in nematodes outside the genus Caenorhabditis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 90 citations in OpenAlex.
- A novel, essential trans-splicing protein connects the nematode SL1 snRNP to the CBC-ARS2 complex.Nucleic acids research · 2022Article
- Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphisms.Nature communications · 2022Article
- A telomere-to-telomere assembly of Oscheius tipulae and the evolution of rhabditid nematode chromosomes.G3 (Bethesda, Md.) · 2021Article
- Resolution of polycistronic RNA by SL2RNA (New York, N.Y.) · 2020Article
- mRNA Editing, Processing and Quality Control inGenetics · 2020Review
- On the Possibility of an Early Evolutionary Origin for the Spliced Leader Trans-Splicing.Journal of molecular evolution · 2017Review
- Oscheius tipulae in Italy: Evidence of an Alien Isolate in the Integral Natural Reserve of Montecristo Island (Tuscany).Journal of nematology · 2016Article
- Article
- Operons are a conserved feature of nematode genomes.Genetics · 2014Article
- Trans-splicing and operons in C. elegans.WormBook : the online review of C. elegans biology · 2012Review
- Polycistronic pre-mRNA processing in vitro: snRNP and pre-mRNA role reversal in trans-splicing.Genes & development · 2010Article
- SL2-like spliced leader RNAs in the basal nematode Prionchulus punctatus: New insight into the evolution of nematode SL2 RNAs.RNA (New York, N.Y.) · 2010Article
- Article
- Loss of the insulator protein CTCF during nematode evolution.BMC molecular biology · 2009Article
- Oscheius tipulae as an example of eEF1A gene diversity in nematodes.Journal of molecular evolution · 2008Article
- Evolutionary dynamics of nematode operons: easy come, slow go.Genome research · 2008Article
- A novel family of C. elegans snRNPs contains proteins associated with trans-splicing.RNA (New York, N.Y.) · 2007Article
- Operon conservation and the evolution of trans-splicing in the phylum Nematoda.PLoS genetics · 2006Article
- On the paucity of duplicated genes in Caenorhabditis elegans operons.Journal of molecular evolution · 2006Article
- Spliced-leader RNA trans splicing in a chordate, Oikopleura dioica, with a compact genome.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The genomes of most eukaryotes are composed of genes arranged on the chromosomes without regard to function, with each gene transcribed from a promoter at its 5' end. However, the genome of the free-living nematode Caenorhabditis elegans contains numerous polycistronic clusters similar to bacterial operons in which the genes are transcribed sequentially from a single promoter at the 5' end of the cluster. The resulting polycistronic pre-mRNAs are processed into monocistronic mRNAs by conventional 3' end formation, cleavage, and polyadenylation, accompanied by trans-splicing with a specialized spliced leader (SL), SL2. To determine whether this mode of gene organization and expression, apparently unique among the animals, occurs in other species, we have investigated genes in a distantly related free-living rhabditid nematode in the genus Dolichorhabditis (strain CEW1). We have identified both SL1 and SL2 RNAs in this species. In addition, we have sequenced a Dolichorhabditis genomic region containing a gene cluster with all of the characteristics of the C. elegans operons. We show that the downstream gene is trans-spliced to SL2. We also present evidence that suggests that these two genes are also clustered in the C. elegans and Caenorhabditis briggsae genomes. Thus, it appears that the arrangement of genes in operons pre-dates the divergence of the genus Caenorhabditis from the other genera in the family Rhabditidae, and may be more widespread than is currently appreciated.
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