ArticleProceedings of the National Academy of Sciences of the United States of America1997
RanGTP-mediated nuclear export of karyopherin alpha involves its interaction with the nucleoporin Nup153.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 65 citations in OpenAlex.
- Advances in the understanding of nuclear pore complexes in human diseases.Journal of cancer research and clinical oncology · 2024Review
- Spatial Visualization of A-to-I Editing in Cells Using Endonuclease V Immunostaining Assay (EndoVIA).ACS central science · 2024Article
- Exploring Cellular Gateways: Unraveling the Secrets of Disordered Proteins within Live Nuclear Pores.Research square · 2024Article
- Crystallographic data of an importin-α3 dimer in which the two protomers are bridged by a bipartite nuclear localization signal.Data in brief · 2023Article
- Crosstalk between nucleocytoplasmic trafficking and the innate immune response to viral infection.The Journal of biological chemistry · 2021Review
- Article
- Importin α: functions as a nuclear transport factor and beyond.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- Viral subversion of the nuclear pore complex.Viruses · 2013Review
- The Nup153-Nup50 protein interface and its role in nuclear import.The Journal of biological chemistry · 2012Article
- Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2.Nucleus (Austin, Tex.) · 2012Article
- The nuclear pore complex and nuclear transport.Cold Spring Harbor perspectives in biology · 2010Review
- Avian reovirus sigmaA localizes to the nucleolus and enters the nucleus by a nonclassical energy- and carrier-independent pathway.Journal of virology · 2009Article
- In vitro nuclear interactome of the HIV-1 Tat protein.Retrovirology · 2009Article
- Multiple conserved domains of the nucleoporin Nup124p and its orthologs Nup1p and Nup153 are critical for nuclear import and activity of the fission yeast Tf1 retrotransposon.Molecular biology of the cell · 2007Article
- Versatility at the nuclear pore complex: lessons learned from the nucleoporin Nup153.Chromosoma · 2005Review
- Dissection of a novel nuclear localization signal in open reading frame 29 of varicella-zoster virus.Journal of virology · 2005Article
- Importin-alpha promotes passage through the nuclear pore complex of human immunodeficiency virus type 1 Vpr.Journal of virology · 2005Article
- The RNA binding domain within the nucleoporin Nup153 associates preferentially with single-stranded RNA.RNA (New York, N.Y.) · 2004Article
- Inhibition of nucleo-cytoplasmic trafficking by RNA viruses: targeting the nuclear pore complex.Virus research · 2003Review
- Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex.Molecular biology of the cell · 2003Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using binding assays, we discovered an interaction between karyopherin alpha2 and the nucleoporin Nup153 and mapped their interacting domains. We also isolated a 15-kDa tryptic fragment of karyopherin beta1, termed beta1*, that contains a determinant for binding to the peptide repeat containing nucleoporin Nup98. In an in vitro assay in which export of endogenous nuclear karyopherin alpha from nuclei of digitonin-permeabilized cells was quantitatively monitored by indirect immunofluorescence with anti-karyopherin alpha antibodies, we found that karyopherin alpha export was stimulated by added GTPase Ran, required GTP hydrolysis, and was inhibited by wheat germ agglutinin. RanGTP-mediated export of karyopherin alpha was inhibited by peptides representing the interacting domains of Nup153 and karyopherin alpha2, indicating that the binding reactions detected in vitro are physiologically relevant and verifying our mapping data. Moreover, beta1*, although it inhibited import, did not inhibit export of karyopherin alpha. Hence, karyopherin alpha import into and export from nuclei are asymmetric processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.