ArticleMolecular and cellular biology1997
Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 62 citations in OpenAlex.
- Article
- Single-molecule Microscopy Reveals That TFIIE Subunits Dynamically Interact With Preinitiation Complexes in a Manner Impacted by TFIIH.Journal of molecular biology · 2025Article
- The general transcription factors (GTFs) of RNA polymerase II and their roles in plant development and stress responses.Critical reviews in biochemistry and molecular biology · 2024Review
- Mechanisms and Functions of the RNA Polymerase II General Transcription Machinery during the Transcription Cycle.Biomolecules · 2024Review
- Direct binding of TFEα opens DNA binding cleft of RNA polymerase.Nature communications · 2020Article
- The hRPC62 subunit of human RNA polymerase III displays helicase activity.Nucleic acids research · 2019Article
- Article
- Structural basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2015Review
- Architecture of the RNA polymerase II preinitiation complex and mechanism of ATP-dependent promoter opening.Nature structural & molecular biology · 2012Article
- Yeast Swd2 is essential because of antagonism between Set1 histone methyltransferase complex and APT (associated with Pta1) termination factor.The Journal of biological chemistry · 2012Article
- Sub1 and RPA associate with RNA polymerase II at different stages of transcription.Molecular cell · 2011Article
- The initiation factor TFE and the elongation factor Spt4/5 compete for the RNAP clamp during transcription initiation and elongation.Molecular cell · 2011Article
- Selective depletion of Sulfolobus solfataricus transcription factor E under heat shock conditions.Journal of bacteriology · 2010Article
- Structural insight into the TFIIE-TFIIH interaction: TFIIE and p53 share the binding region on TFIIH.The EMBO journal · 2008Article
- p53 and TFIIEalpha share a common binding site on the Tfb1/p62 subunit of TFIIH.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Article
- Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly.The EMBO journal · 2003Article
- An early function during transcription for the yeast mRNA export factor Dbp5p/Rat8p suggested by its genetic and physical interactions with transcription factor IIH components.Molecular biology of the cell · 2003Article
- Messenger RNAs are recruited for nuclear export during transcription.Genes & development · 2001Article
- TFE, an archaeal transcription factor in Methanobacterium thermoautotrophicum related to eucaryal transcription factor TFIIEalpha.Journal of bacteriology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Biochemical analysis of proteins necessary for transcription initiation by eukaryotic RNA polymerase II (pol II) has identified transcription factor IIE (TFIIE) as an essential component of the reaction. To better understand the role of TFIIE in transcription, we isolated conditional alleles of TFA1, the gene encoding the large subunit of TFIIE in the yeast Saccharomyces cerevisiae. The mutant Tfa1 proteins fall into two classes. The first class causes thermosensitive growth due to single amino acid substitutions of the cysteines comprising the Zn-binding motif. The second mutant class is made up of proteins that are C-terminally truncated and that cause a cold-sensitive growth phenotype. The behavior of these mutants suggests that Tfa1p possesses at least two domains with genetically distinct functions. The mutations in the Zn-binding motif do not affect the mutant protein's stability at the nonpermissive temperature or its ability to associate with the small subunit of TFIIE. Our studies further determined that wild-type TFIIE can bind to single-stranded DNA in vitro. However, this property is unaffected in the mutant TFIIE complexes. Finally, we have demonstrated the biological importance of TFIIE in pol II-mediated transcription by depleting the Tfa1 protein from the cells and observing a concomitant decrease in total poly(A)+ mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.