ArticleMolecular and cellular biology1997
Core binding factor cannot synergistically activate the myeloperoxidase proximal enhancer in immature myeloid cells without c-Myb.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 95 citations in OpenAlex.
- miR-150 Regulates Memory CD8 T Cell Differentiation via c-Myb.Cell reports · 2017Article
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- A unifying gene signature for adenoid cystic cancer identifies parallel MYB-dependent and MYB-independent therapeutic targets.Oncotarget · 2014Article
- Impaired adult myeloid progenitor CMP and GMP cell function in conditional c-myb-knockout mice.Cell cycle (Georgetown, Tex.) · 2012Article
- Integrated genome-wide chromatin occupancy and expression analyses identify key myeloid pro-differentiation transcription factors repressed by Myb.Nucleic acids research · 2011Article
- Myb proteins: angels and demons in normal and transformed cells.Frontiers in bioscience (Landmark edition) · 2011Review
- A SUMO-regulated activation function controls synergy of c-Myb through a repressor-activator switch leading to differential p300 recruitment.Nucleic acids research · 2010Article
- Overexpression of an isoform of AML1 in acute leukemia and its potential role in leukemogenesis.Leukemia · 2009Article
- Predicting combinatorial binding of transcription factors to regulatory elements in the human genome by association rule mining.BMC bioinformatics · 2007Article
- The multi-functional cellular adhesion molecule CD44 is regulated by the 8;21 chromosomal translocation.Leukemia · 2007Article
- Article
- ALY is a common coactivator of RUNX1 and c-Myb on the type B leukemogenic virus enhancer.Journal of virology · 2007Article
- Article
- Roles of HIPK1 and HIPK2 in AML1- and p300-dependent transcription, hematopoiesis and blood vessel formation.The EMBO journal · 2006Article
- v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter.Molecular and cellular biology · 2005Article
- Williams-Beuren syndrome critical region-5/non-T-cell activation linker: a novel target gene of AML1/ETO.Oncogene · 2004Article
- Article
- Identification of genes that synergize with Cbfb-MYH11 in the pathogenesis of acute myeloid leukemia.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Specific protein redirection as a transcriptional therapy approach for t(8;21) leukemia.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Cell cycle inhibition mediated by the outer surface of the C/EBPalpha basic region is required but not sufficient for granulopoiesis.Oncogene · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The myeloperoxidase (MPO) gene is transcribed specifically in immature myeloid cells and is regulated in part by a 414-bp proximal enhancer. Mutation of a core binding factor (CBF)-binding site at -288 decreased enhancer activity 30-fold in 32D cl3 myeloid cells cultured in granulocyte colony-stimulating factor (G-CSF). A novel functional analysis, linking the CBF-binding site to an enhancer deletion series, located at -147 an evolutionarily conserved c-Myb-binding site which was required for optimal enhancer activity and synergy with CBF in 32D cells. These sites cooperated in isolation and independent of a precise spacing. Deletional analysis carried out in the absence of the c-Myb-binding site at -147 located at -301 a second c-Myb-binding site which also synergized with CBF to activate the enhancer. A GA-rich region at -162 contributed to cooperation with CBF when the adjacent c-Myb-binding site was intact. Mutation of both c-Myb-binding sites in the context of the entire enhancer greatly impaired activation by endogenous CBF in 32D cells. Similarly, activation by c-Myb was impaired in constructs lacking the CBF-binding site. CBF and c-Myb were required for induction of MPO proximal enhancer activity when 32D cells differentiated in response to G-CSF. A fusion protein containing the Gal4 DNA-binding domain and the AML-1B activation domain, amino acids 216 to 480, activated transcription alone and cooperatively with c-Myb in nonmyeloid CV-1 cells. Determining how CBF and c-Myb synergize in myeloid cells might contribute to our understanding of leukemogenesis by the AML1-ETO, AML1-MDS1, CBFbeta-SMMHC, and v-Myb oncoproteins.
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