ArticleMolecular and cellular biology1997
Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 95 citations in OpenAlex.
- A connection between the ribosome and two S. pombe tRNA modification mutants subject to rapid tRNA decay.PLoS genetics · 2024Article
- The ribosome assembly factor Nop53 controls association of the RNA exosome with pre-60S particles in yeast.The Journal of biological chemistry · 2019Article
- Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae.Nucleic acids research · 2018Article
- Article
- Disruption of ribosome assembly in yeast blocks cotranscriptional pre-rRNA processing and affects the global hierarchy of ribosome biogenesis.RNA (New York, N.Y.) · 2016Article
- Signaling crosstalk between the mTOR complexes.Translation (Austin, Tex.) · 2014Review
- Kinetic analysis demonstrates a requirement for the Rat1 exonuclease in cotranscriptional pre-rRNA cleavage.PloS one · 2014Article
- Ribosome biogenesis in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- Ribosome biogenesis requires a highly diverged XRN family 5'->3' exoribonuclease for rRNA processing in Trypanosoma brucei.RNA (New York, N.Y.) · 2013Article
- Temporal analysis of the magnaporthe oryzae proteome during conidial germination and cyclic AMP (cAMP)-mediated appressorium formation.Molecular & cellular proteomics : MCP · 2013Article
- Yeast polypeptide exit tunnel ribosomal proteins L17, L35 and L37 are necessary to recruit late-assembling factors required for 27SB pre-rRNA processing.Nucleic acids research · 2013Article
- Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae.Nucleic acids research · 2012Article
- Saccharomyces cerevisiae ribosomal protein L26 is not essential for ribosome assembly and function.Molecular and cellular biology · 2012Article
- Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts.PloS one · 2012Article
- The central core region of yeast ribosomal protein L11 is important for subunit joining and translational fidelity.Molecular genetics and genomics : MGG · 2011Article
- The NIP7 protein is required for accurate pre-rRNA processing in human cells.Nucleic acids research · 2011Article
- The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing.PloS one · 2011Article
- The essential nucleolar yeast protein Nop8p controls the exosome function during 60S ribosomal subunit maturation.PloS one · 2011Article
- Ribosomal protein L35 is required for 27SB pre-rRNA processing in Saccharomyces cerevisiae.Nucleic acids research · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae temperature-sensitive (ts) allele nip7-1 exhibits phenotypes associated with defects in the translation apparatus, including hypersensitivity to paromomycin and accumulation of halfmer polysomes. The cloned NIP7+ gene complemented the nip7-1 ts growth defect, the paromomycin hypersensitivity, and the halfmer defect. NIP7 encodes a 181-amino-acid protein (21 kDa) with homology to predicted products of open reading frames from humans, Caenorhabditis elegans, and Arabidopsis thaliana, indicating that Nip7p function is evolutionarily conserved. Gene disruption analysis demonstrated that NIP7 is essential for growth. A fraction of Nip7p cosedimented through sucrose gradients with free 60S ribosomal subunits but not with 80S monosomes or polysomal ribosomes, indicating that it is not a ribosomal protein. Nip7p was found evenly distributed throughout the cytoplasm and nucleus by indirect immunofluorescence; however, in vivo localization of a Nip7p-green fluorescent protein fusion protein revealed that a significant amount of Nip7p is present inside the nucleus, most probably in the nucleolus. Depletion of Nip7-1p resulted in a decrease in protein synthesis rates, accumulation of halfmers, reduced levels of 60S subunits, and, ultimately, cessation of growth. Nip7-1p-depleted cells showed defective pre-rRNA processing, including accumulation of the 35S rRNA precursor, presence of a 23S aberrant precursor, decreased 20S pre-rRNA levels, and accumulation of 27S pre-rRNA. Delayed processing of 27S pre-rRNA appeared to be the cause of reduced synthesis of 25S rRNA relative to 18S rRNA, which may be responsible for the deficit of 60S subunits in these cells.
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