ArticleMolecular and cellular biology1997
The murine Sim-2 gene product inhibits transcription by active repression and functional interference.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 92 citations in OpenAlex.
- Interaction patterns of methoprene-tolerant and germ cell-expressedFrontiers in molecular biosciences · 2023Article
- Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes.The Biochemical journal · 2022Article
- The Mammary Gland: Basic Structure and Molecular Signaling during Development.International journal of molecular sciences · 2022Review
- Single-minded 2 is required for left-right asymmetric stomach morphogenesis.Development (Cambridge, England) · 2021Article
- The Participation of the Intrinsically Disordered Regions of the bHLH-PAS Transcription Factors in Disease Development.International journal of molecular sciences · 2021Article
- Genomic Analyses Reveal Adaptation to Hot Arid and Harsh Environments in Native Chickens of China.Frontiers in genetics · 2020Article
- bHLH-PAS Proteins: Their Structure and Intrinsic Disorder.International journal of molecular sciences · 2019Review
- Cancer/Testis Antigen PASD1 Silences the Circadian Clock.Molecular cell · 2015Article
- Dioxin exposure blocks lactation through a direct effect on mammary epithelial cells mediated by the aryl hydrocarbon receptor repressor.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- Regulated transcription of human matrix metalloproteinase 13 (MMP13) and interleukin-1β (IL1B) genes in chondrocytes depends on methylation of specific proximal promoter CpG sites.The Journal of biological chemistry · 2013Article
- Sim2 prevents entry into the myogenic program by repressing MyoD transcription during limb embryonic myogenesis.Development (Cambridge, England) · 2012Article
- Article
- Epithelial-mesenchymal transition in cancer: parallels between normal development and tumor progression.Journal of mammary gland biology and neoplasia · 2010Review
- Article
- Slow transition between two beta-strand registers is dictated by protein unfolding.Journal of the American Chemical Society · 2009Article
- The active form of human aryl hydrocarbon receptor (AHR) repressor lacks exon 8, and its Pro 185 and Ala 185 variants repress both AHR and hypoxia-inducible factor.Molecular and cellular biology · 2009Article
- ARNT PAS-B has a fragile native state structure with an alternative beta-sheet register nearby in sequence space.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Disruption of period gene expression alters the inductive effects of dioxin on the AhR signaling pathway in the mouse liver.Toxicology and applied pharmacology · 2009Article
- The bHLH/Per-Arnt-Sim transcription factor SIM2 regulates muscle transcript myomesin2 via a novel, non-canonical E-box sequence.Nucleic acids research · 2008Article
- Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: potential mechanisms of action.Breast cancer research : BCR · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila single-minded (Dsim) gene encodes a master regulatory protein involved in cell fate determination during midline development. This protein is a member of a rapidly expanding family of gene products possessing basic helix-loop-helix (bHLH) and hydrophobic PAS (designated a conserved region among PER, ARNT [aryl hydrocarbon receptor nuclear translocator] and SIM) protein association domains. Members of this family function as central transcriptional regulators in cellular differentiation and in the response to environmental stimuli such as xenobiotics and hypoxia. We have previously identified a murine member of this family, called mSim-2, showing sequence homology to the bHLH and PAS domains of Dsim. Immunoprecipitation experiments with recombinant proteins indicate that mSIM-2 associates with the arnt gene product. In the present work, by using fine-structure mapping we found that the HLH and PAS motifs of both proteins are required for optimal association. Forced expression of GAL4/mSIM-2 fusion constructs in mammalian cells demonstrated the presence of two separable repression domains within the carboxy terminus of mSIM-2. We found that mSIM-2 is capable of repressing ARNT-mediated transcriptional activation in a mammalian two-hybrid system. This effect (i) is dependent on the ability of mSIM-2 and ARNT to heterodimerize, (ii) is dependent on the presence of the mSIM-2 carboxy-terminal repression domain, and (iii) is not specific to the ARNT activation domain. These results suggest that mSIM-2 repression activity can dominantly override the activation potential of adjacent transcription factors. We also demonstrated that mSIM-2 can functionally interfere with hypoxia-inducible factor 1alpha (HIF-1alpha)/ARNT transcription complexes, providing a second mechanism by which mSIM-2 may inhibit transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.