ArticleMolecular and cellular biology1997
Ribosome stalling is responsible for arginine-specific translational attenuation in Neurospora crassa.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers, 1 of them a synthesis that pooled it.
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Who cites it
64 citing papers in PubMed, 1 synthesis or guideline pooled it, 124 citations in OpenAlex.
- Systematic Review of Plant Ribosome Heterogeneity and Specialization.Frontiers in plant science · 2020Pooled it
- Mechanism of ribosome stalling by the AMD1 C-terminal tail arrest peptide.Science advances · 2026Article
- Control of mRNA fate by its encoded nascent polypeptide.Molecular cell · 2023Review
- Translational regulation by uORFs and start codon selection stringency.Genes & development · 2023Review
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Translational autoregulation of the S. cerevisiae high-affinity polyamine transporter Hol1.Molecular cell · 2021Article
- Substrate-mediated regulation of the arginine transporter of Toxoplasma gondii.PLoS pathogens · 2021Article
- Smart-ORF: a single-molecule method for accessing ribosome dynamics in both upstream and main open reading frames.Nucleic acids research · 2021Article
- Conserved Upstream Open Reading Frame Nascent Peptides That Control Translation.Annual review of genetics · 2020Review
- Exhaustive identification of conserved upstream open reading frames with potential translational regulatory functions from animal genomes.Scientific reports · 2020Article
- An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics.Journal of visualized experiments : JoVE · 2020Article
- Comprehensive genome-wide identification of angiosperm upstream ORFs with peptide sequences conserved in various taxonomic ranges using a novel pipeline, ESUCA.BMC genomics · 2020Article
- Reverse genetics-based biochemical studies of the ribosomal exit tunnel constriction region in eukaryotic ribosome stalling: spatial allocation of the regulatory nascent peptide at the constriction.Nucleic acids research · 2020Article
- DeepShape: estimating isoform-level ribosome abundance and distribution with Ribo-seq data.BMC bioinformatics · 2019Article
- Impacts of uORF codon identity and position on translation regulation.Nucleic acids research · 2019Article
- A dual role of the ribosome-bound chaperones RAC/Ssb in maintaining the fidelity of translation termination.Nucleic acids research · 2019Article
- Magnesium-sensitive upstream ORF controls PRL phosphatase expression to mediate energy metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Translation Termination and Ribosome Recycling in Eukaryotes.Cold Spring Harbor perspectives in biology · 2018Review
- Polyamine Control of Translation Elongation Regulates Start Site Selection on Antizyme Inhibitor mRNA via Ribosome Queuing.Molecular cell · 2018Article
- The cell free protein synthesis system from the model filamentous fungus Neurospora crassa.Methods (San Diego, Calif.) · 2018Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Neurospora crassa arg-2 upstream open reading frame (uORF) plays a role in negative arginine-specific translational regulation. Primer extension inhibition analyses of arg-2 uORF-containing RNA translated in a cell-free system in which arginine-specific regulation was retained revealed "toeprints" corresponding to ribosomes positioned at the uORF initiation and termination codons and at the downstream initiation codon. At high arginine concentrations, the toeprint signal corresponding to ribosomes at the uORF termination codon rapidly increased; a new, broad toeprint that represents additional ribosomes stalled on the uORF appeared 21 to 30 nucleotides upstream of this site; and the toeprint signal corresponding to ribosomes at the downstream initiation codon decreased. These data suggest that arginine increases ribosomal stalling and thereby decreases translation from the downstream initiation codon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.