ArticleGenes & development1997
Alpha2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III.
Article in Genes & development, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 51 citations in OpenAlex.
- Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III.PLoS genetics · 2023Article
- A Sir2-regulated locus control region in the recombination enhancer of Saccharomyces cerevisiae specifies chromosome III structure.PLoS genetics · 2019Article
- Mating-type switching by homology-directed recombinational repair: a matter of choice.Current genetics · 2019Review
- Chromosome-refolding model of mating-type switching in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Donor Preference Meets Heterochromatin: Moonlighting Activities of a Recombinational Enhancer in Saccharomyces cerevisiae.Genetics · 2016Article
- The Conformation of Yeast Chromosome III Is Mating Type Dependent and Controlled by the Recombination Enhancer.Cell reports · 2015Article
- Differential chromosome conformations as hallmarks of cellular identity revealed by mathematical polymer modeling.PLoS computational biology · 2015Article
- Two portable recombination enhancers direct donor choice in fission yeast heterochromatin.PLoS genetics · 2013Article
- Review
- Regulation of budding yeast mating-type switching donor preference by the FHA domain of Fkh1.PLoS genetics · 2012Article
- Evolutionary erosion of yeast sex chromosomes by mating-type switching accidents.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Sir2 silences gene transcription by targeting the transition between RNA polymerase II initiation and elongation.Molecular and cellular biology · 2008Article
- Cell cycle-dependent regulation of Saccharomyces cerevisiae donor preference during mating-type switching by SBF (Swi4/Swi6) and Fkh1.Molecular and cellular biology · 2006Article
- The DNA repair protein yKu80 regulates the function of recombination enhancer during yeast mating type switching.Molecular and cellular biology · 2005Article
- Yeast recombination enhancer is stimulated by transcription activation.Molecular and cellular biology · 2005Article
- Genomic dissection of the cell-type-specification circuit in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Global chromatin structure of 45,000 base pairs of chromosome III in a- and alpha-cell yeast and during mating-type switching.Molecular and cellular biology · 2004Article
- Article
- Mating type-dependent constraints on the mobility of the left arm of yeast chromosome III.The Journal of cell biology · 2004Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homothallic strains of Saccharomyces cerevisiae can change mating type as often as every generation by replacing the allele at the MAT locus with a copy of mating type information present at one of two storage loci, HML and HMR, located on either end of chromosome III. Selection of the appropriate donor locus is dictated by a mating type-specific repressor protein, alpha2p: Cells containing alpha2p select HMR, whereas those lacking alpha2p select HML. As a repressor protein, alpha2p binds to DNA cooperatively with the transcriptional activator Mcm1p. Here we show that two alpha2p/Mcm1p-binding sites, DPS1 and DPS2, control donor selection. DPS1 and DPS2 are located approximately 30 kb from the left arm of chromosome III, well removed from HML, HMR, and MAT. Precise deletion of only DPS1 and DPS2 results in random selection of donor loci and in a cells without affecting selection in alpha cells. Reciprocally, deletion of only the alpha2p binding segments in each of these two sites results in selection of the wrong donor loci in alpha cells without affecting preference in a cells. These results suggest that Mcm1p, bound to these two sites in the absence of alpha2p, activates HML as donor. Binding of alpha2p blocks the ability of Mcm1p bound to DPS1 and DPS2 to activate HML, resulting in default selection of HMR as donor. DPS1 and DPS2 also regulate expression of several noncoding RNAs, although deletion of at least one of these RNA loci does not affect donor preference. This suggests that transcriptional activation, rather than transcription of a specific product, is the initiating event in activating the left arm of chromosome III for donor selection.
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