ArticleThe Biochemical journal1997
Parallel changes in nuclear and cytosolic calcium in mouse pancreatic beta-cells.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 27 citations in OpenAlex.
- Sex differences impact the pancreatic response to chronic immobilization stress in rats.Cell stress & chaperones · 2021Article
- Impact of chronic exercise on counteracting chronic stress-induced functional and morphological pancreatic changes in male albino rats.Cell stress & chaperones · 2019Article
- Nuclear pores enable sustained perinuclear calcium oscillations.BMC systems biology · 2016Article
- Imaging beta-cell mass and function in situ and in vivo.Journal of molecular medicine (Berlin, Germany) · 2013Review
- Nuclear Ca2+ sparks and waves mediated by inositol 1,4,5-trisphosphate receptors in neonatal rat cardiomyocytes.Cell calcium · 2008Article
- Circularly permuted green fluorescent proteins engineered to sense Ca2+.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons.Biophysical journal · 1999Article
- Hydrogen peroxide-induced DNA damage is independent of nuclear calcium but dependent on redox-active ions.The Biochemical journal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the neuroendocrine pancreatic beta-cell, elevations in intracellular Ca2+ lead to insulin secretion and the initiation of gene transcription. However, the relationship between cytosolic and nuclear Ca2+ in these cells is unknown. The Ca2+ permeability of the nuclear membrane would therefore determine if Ca2+ could play a direct role in Ca2+-dependent nuclear processes. Using confocal fluorescence microscopy with the ratiometric Ca2+ indicator indo-1 and carefully correcting for compartmentalized indicator, we now demonstrate that there is no difference between the nuclear Ca2+ concentration and the cytosolic Ca2+ concentration ([Ca2+]c) in the resting beta-cell. Slow Ca2+ oscillations induced by glucose, fast oscillations induced by glucagon-like peptide-1 and responses to potassium and carbachol all indicate that changes in cytosolic Ca2+ are reflected within the nucleus. We conclude that there are no restrictions on Ca2+ entry into the nucleus of the pancreatic beta-cell subsequent to increases in [Ca2+]c. This implies that any signal involved in increasing [Ca2+]c, and thereby insulin release, may also promote nuclear Ca2+-induced gene transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.