ArticleThe Journal of cell biology1997
Gelsolin modulates phospholipase C activity in vivo through phospholipid binding.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 93 citations in OpenAlex.
- Exploring the role of gelsolin and high-density lipoproteins in autoimmune diseases: spotlight on rheumatoid arthritis and systemic lupus erythematosus.Oxford open immunology · 2026Review
- Regulation of Mitochondrial Function by the Actin Cytoskeleton.Frontiers in cell and developmental biology · 2021Review
- Article
- Phosphoinositide Binding Inhibits Actin Crosslinking and Polymerization by Palladin.Journal of molecular biology · 2016Article
- Gelsolin and ceruloplasmin as potential predictive biomarkers for cervical cancer by 2D-DIGE proteomics analysis.Pathology oncology research : POR · 2014Article
- Role and regulation of sperm gelsolin prior to fertilization.The Journal of biological chemistry · 2010Article
- Critical role for the catalytic activity of phospholipase C-gamma1 in epidermal growth factor-induced cell migration.Biochemical and biophysical research communications · 2010Article
- The SH3 domain, but not the catalytic domain, is required for phospholipase C-gamma1 to mediate epidermal growth factor-induced mitogenesis.Biochemical and biophysical research communications · 2010Article
- Phospholipase C-gamma1 is required for the epidermal growth factor receptor-induced squamous cell carcinoma cell mitogenesis.Biochemical and biophysical research communications · 2010Article
- Phosphatidylinositol-4-phosphate 5-kinase 1alpha mediates extracellular calcium-induced keratinocyte differentiation.Molecular biology of the cell · 2009Article
- Accelerators, Brakes, and Gears of Actin Dynamics in Dendritic Spines.The open neuroscience journal · 2009Article
- Duplicated gelsolin family genes in zebrafish: a novel scinderin-like gene (scinla) encodes the major corneal crystallin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2007Article
- Separation of insulin signaling into distinct GLUT4 translocation and activation steps.Molecular and cellular biology · 2004Article
- The role of the actin cytoskeleton in plant cell signaling.The New phytologist · 2004Review
- Gelsolin suppresses tumorigenicity through inhibiting PKC activation in a human lung cancer cell line, PC10.British journal of cancer · 2003Article
- Agonist-induced PIP(2) hydrolysis inhibits cortical actin dynamics: regulation at a global but not at a micrometer scale.Molecular biology of the cell · 2002Article
- Phosphoinositides regulate membrane-dependent actin assembly by latex bead phagosomes.Molecular biology of the cell · 2002Article
- Phosphatidylinositol 4,5-bisphosphate modifies tubulin participation in phospholipase Cbeta1 signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Sparks and puffs in oligodendrocyte progenitors: cross talk between ryanodine receptors and inositol trisphosphate receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Phosphatidylinositol 4,5-bisphosphate induces actin stress-fiber formation and inhibits membrane ruffling in CV1 cells.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Gelsolin and CapG are actin regulatory proteins that remodel the cytoskeleton in response to phosphatidylinositol 4,5-bisphosphate (PIP2) and Ca2+ during agonist stimulation. A physiologically relevant rise in Ca2+ increases their affinity for PIP2 and can promote significant interactions with PIP2 in activated cells. This may impact divergent PIP2- dependent signaling processes at the level of substrate availability. We found that CapG overexpression enhances PDGF-stimulated phospholipase Cgamma (PLCgamma) activity (Sun, H.-q., K. Kwiatkowska, D.C. Wooten, and H.L. Yin. 1995. J. Cell Biol. 129:147-156). In this paper, we examined the ability of gelsolin and CapG to compete with another PLC for PIP2 in live cells, in semiintact cells, and in vitro. We found that CapG and gelsolin overexpression profoundly inhibited bradykinin-stimulated PLCbeta. Inhibition occurred at or after the G protein activation step because overexpression also reduced the response to direct G protein activation with NaF. Bradykinin responsiveness was restored after cytosolic proteins, including gelsolin, leaked out of the overexpressing cells. Conversely, exogenous gelsolin added to permeabilized cells inhibited response in a dose-dependent manner. The washout and addback experiments clearly establish that excess gelsolin is the primary cause of PLC inhibition in cells. In vitro experiments showed that gelsolin and CapG stimulated as well as inhibited PLCbeta, and only gelsolin domains containing PIP2-binding sites were effective. Inhibition was mitigated by increasing PIP2 concentration in a manner consistent with competition between gelsolin and PLCbeta for PIP2. Gelsolin and CapG also had biphasic effects on tyrosine kinase- phosphorylated PLCgamma, although they inhibited PLCgamma less than PLCbeta. Our findings indicate that as PIP2 level and availability change during signaling, cross talk between PIP2-regulated proteins provides a selective mechanism for positive as well as negative regulation of the signal transduction cascade.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.