ArticleThe Journal of physiology1997
Receptors couple to L-type calcium channels via distinct Go proteins in rat neuroendocrine cell lines.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 45 citations in OpenAlex.
- Ionic mechanisms in pancreatic β cell signaling.Cellular and molecular life sciences : CMLS · 2014Review
- Augmented glucose-induced insulin release in mice lacking G(o2), but not G(o1) or G(i) proteins.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Coordinated control of sensitivity by two splice variants of Gα(o) in retinal ON bipolar cells.The Journal of general physiology · 2010Article
- Pituitary somatostatin receptor signaling.Trends in endocrinology and metabolism: TEM · 2010Review
- Immunohistochemical localization of G protein betagamma subunits in the lateral wall of the rat cochlea.Journal of anatomy · 2006Article
- Group III metabotropic glutamate receptors and exocytosed protons inhibit L-type calcium currents in cones but not in rods.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Light response of retinal ON bipolar cells requires a specific splice variant of Galpha(o).The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Cocaine- and amphetamine-regulated transcript peptide modulation of voltage-gated Ca2+ signaling in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Role of the D2 dopamine receptor in molecular adaptation to chronic hypoxia in PC12 cells.Pflugers Archiv : European journal of physiology · 1999Article
- Posttranslational modification of Galphao1 generates Galphao3, an abundant G protein in brain.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The present study examines the hypothesis of G protein subtype selectivity in receptor-induced inhibition of calcium channel currents (ICa) in the insulin-secreting RINm5F and pituitary GH3 rat cell lines. Specificity of receptor coupling to G proteins was studied by infusion of purified G alpha isoforms into cells via a patch pipette. 2. In RINm5F cells, the neuropeptide galanin inhibited dihydropyridine (DHP)- and omega-conotoxin-sensitive components of ICa and slowed down their activation kinetics. In GH3 cells, DHP-sensitive ICa was inhibited by galanin, as well as by somatostatin and carbachol. Agonist-induced ICa inhibition was suppressed by pertussis toxin (PTX) pretreatment of the cells. In PTX-pretreated cells of either cell line, the response to galanin was restored only by the G alpha o1 subunit. Following PTX treatment of GH3 cells, only the G alpha o1 subunit restored carbachol-induced inhibition of ICa, whereas only the G alpha o2 subunit restored somatostatin-induced inhibition of ICa. G(i) subtypes had no effect on ICa inhibition. 3. Both cell lines expressed two distinct immunoreactive Go proteins. Whereas in RINm5F cell membranes Go1 was found to be the predominant isoform, we detected more Go2 than Go1 in GH3 cell membranes. Nevertheless, all agonists stimulated incorporation of the photoreactive GTP analogue [alpha-32P]GTP azidoanilide into both G(o) isoforms. 4. The results indicate that the same Go subtype, i.e. Go1, mediates galanin-induced inhibition of ICa in both cell lines and that the Go subtype specificity of receptor-G protein coupling is confined to intact cells.
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