ArticleJournal of virology1997
cis elements that contribute to geminivirus transcriptional regulation and the efficiency of DNA replication.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Analysis of bidirectional promoter activity and structure of the large intergenic region (LIR) in mulberry crinkle leaf virus.Plant molecular biology · 2026Article
- Revelation of core promoter elements and bidirectional regulation of Geminivirus genes in Escherichia coli.Virus research · 2026Article
- Structural Modeling of Protein-DNA Interactions Underlying Genome Copy Number Variation in Nanoviruses.Computational and structural biotechnology journal · 2026Article
- Rescue of tomato yellow leaf curl virus mutants harboring heterologous iterons throughJournal of virology · 2025Article
- Molecular characterization of a begomovirus associated with yellow mosaic disease ofVirusdisease · 2025Article
- Transient expression analysis of promoters of okra enation leaf curl virus in Nicotiana benthamiana, cotton and okra plants.Virus genes · 2024Article
- Insights into the Differential Composition of Stem-Loop Structures of Nanoviruses and Their Impacts.Microbiology spectrum · 2023Article
- Weed-infecting viruses in a tropical agroecosystem present different threats to crops and evolutionary histories.PloS one · 2021Article
- Article
- Plant-virus-insect tritrophic interactions: insights into the functions of geminivirus virion-sense strand genes.Proceedings. Biological sciences · 2020Article
- Whitefly HES1 binds to the intergenic region of Tomato yellow leaf curl China virus and promotes viral gene transcription.Virology · 2020Article
- Biology and Interaction of the Natural Occurrence of Distinct Monopartite Begomoviruses Associated With Satellites inFrontiers in microbiology · 2020Article
- Iterons Homologous to Helper Geminiviruses Are Essential for Efficient Replication of Betasatellites.Journal of virology · 2019Article
- Early function of the Abutilon mosaic virus AC2 gene as a replication brake.Journal of virology · 2015Article
- Functional characterization of a strong bi-directional constitutive plant promoter isolated from cotton leaf curl Burewala virus.PloS one · 2015Article
- Molecular characterization of a naturally occurring intraspecific recombinant begomovirus with close relatives widespread in southern Arabia.Virology journal · 2014Article
- Article
- Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus.BMC evolutionary biology · 2012Article
- Characterization of sequence elements from Malvastrum yellow vein betasatellite regulating promoter activity and DNA replication.Virology journal · 2012Article
- The presence of tomato leaf curl Kerala virus AC3 protein enhances viral DNA replication and modulates virus induced gene-silencing mechanism in tomato plants.Virology journal · 2011Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The A genomic component of the geminivirus tomato golden mosaic virus (TGMV) contains a 5' intergenic sequence that includes the overlapping AL61 promoter and positive-strand origin of DNA replication. The TGMV AL1 protein negatively regulates its own transcription and mediates origin recognition by binding to a repeated motif shared by the AL61 promoter and the viral origin. We examined a series of truncated or mutated 5' intergenic regions in transient expression and replication assay to identify other DNA sequences that contribute to TGMV promoter and origin function. These experiments revealed that negative regulation of the AL61 promoter is complex, involving multiple cis-acting sequences and the AL1 and AL4 proteins, which acted through different DNA elements. We also found that mutation of the TATA box motif in the AL61 promoter reduced overall transcriptional activity and AL1-mediated repression, confirming the importance of this sequence in promoter function. Mutation of a G-box consensus sequence was highly detrimental to AL61 transcription and abolished AL1 sensitivity, suggesting that AL1 interferes with transcriptional activation. Cotransfection experiments showed that the TATA box and G-box motif mutations also impaired viral DNA replication in the presence of a wild-type origin but had no effect in its absence, demonstrating that these transcriptional motifs also function as replication efficiency elements.
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