ArticleJournal of virology1997
Poliovirus-encoded protease 2APro cleaves the TATA-binding protein but does not inhibit host cell RNA polymerase II transcription in vitro.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 65 citations in OpenAlex.
- The SARS-CoV-2 SSHHPS Recognized by the Papain-like Protease.ACS infectious diseases · 2021Article
- eIF4G has intrinsic G-quadruplex binding activity that is required for tiRNA function.Nucleic acids research · 2020Article
- Proteolytic cleavage of host proteins by the Group IV viral proteases of Venezuelan equine encephalitis virus and Zika virus.Antiviral research · 2019Article
- Picornaviruses and nuclear functions: targeting a cellular compartment distinct from the replication site of a positive-strand RNA virus.Frontiers in microbiology · 2015Review
- Poliovirus infection induces the co-localization of cellular protein SRp20 with TIA-1, a cytoplasmic stress granule protein.Virus research · 2013Article
- Viral proteinase requirements for the nucleocytoplasmic relocalization of cellular splicing factor SRp20 during picornavirus infections.Journal of virology · 2013Article
- Viral subversion of host functions for picornavirus translation and RNA replication.Future virology · 2012Article
- Escape from transcriptional shutoff during poliovirus infection: NF-κB-responsive genes IκBa and A20.Journal of virology · 2011Article
- The multifaceted poliovirus 2A protease: regulation of gene expression by picornavirus proteases.Journal of biomedicine & biotechnology · 2011Article
- Viral security proteins: counteracting host defences.Nature reviews. Microbiology · 2010Review
- Poliovirus replication requires the N-terminus but not the catalytic Sec7 domain of ArfGEF GBF1.Cellular microbiology · 2010Article
- Stable formation of compositionally unique stress granules in virus-infected cells.Journal of virology · 2010Article
- Viral and host proteins involved in picornavirus life cycle.Journal of biomedical science · 2009Review
- Role for conserved residues of sindbis virus nonstructural protein 2 methyltransferase-like domain in regulation of minus-strand synthesis and development of cytopathic infection.Journal of virology · 2008Article
- Nucleocytoplasmic traffic disorder induced by cardioviruses.Journal of virology · 2006Article
- Shutoff of RNA polymerase II transcription by poliovirus involves 3C protease-mediated cleavage of the TATA-binding protein at an alternative site: incomplete shutoff of transcription interferes with efficient viral replication.Journal of virology · 2005Article
- Differential rescue of poliovirus RNA replication functions by genetically modified RNA polymerase precursors.Journal of virology · 2004Article
- Bidirectional increase in permeability of nuclear envelope upon poliovirus infection and accompanying alterations of nuclear pores.Journal of virology · 2004Article
- Regulation of poliovirus 3C protease by the 2C polypeptide.Journal of virology · 2004Article
- Proteolytic cleavage of the catalytic subunit of DNA-dependent protein kinase during poliovirus infection.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transient expression of the poliovirus-encoded protease 2APro in eukaryotic cells results in inhibition of both cellular transcription and translation. The inhibition of transcription observed in cells expressing 2APro could be due to a primary effect or secondary effect caused by inhibition of translation. Because transcriptional activity of the TATA-binding protein (TBP) is drastically reduced in poliovirus-infected cells, we determined if 2APro is able to cleave TBP in vitro. We demonstrate here that 2APro directly cleaves the single tyrosine-glycine bond at position 34 of TBP. This cleavage is also seen in poliovirus-infected HeLa cells. Surprisingly, despite TBP cleavage 2APro was unable to inhibit RNA polymerase II transcription in vitro. Under similar conditions, however, 2APro inhibited translation of a capped cellular mRNA in vitro. Thus, cleavage of TBP at position 34 does not alter its transcriptional activity in vitro. These results suggest that inhibition of host cell RNA polymerase II transcription seen in cells transiently transfected with 2APro is due to host cell translational shutoff.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.