ArticleJournal of virology1997
Placental trophoblasts resist infection by multiple human immunodeficiency virus (HIV) type 1 variants even with cytomegalovirus coinfection but support HIV replication after provirus transfection.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Placenta models for HIV-1-ART-nicotine comorbidity research: advances and remaining challenges.Cell death discovery · 2026Review
- Roles of TGF-β1 in Viral Infection during Pregnancy: Research Update and Perspectives.International journal of molecular sciences · 2023Review
- Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption.Journal of visualized experiments : JoVE · 2016Article
- Phospholipid scramblase 1 (PLSCR1) in villous trophoblast of the human placenta.Histochemistry and cell biology · 2015Article
- HLA-G 14 bp deletion/insertion polymorphism and mother-to-child transmission of HIV.Tissue antigens · 2014Article
- Role of the placenta in adverse perinatal outcomes among HIV-1 seropositive women.Journal of Nippon Medical School = Nippon Ika Daigaku zasshi · 2013Review
- Antigen-presenting cells represent targets for R5 HIV-1 infection in the first trimester pregnancy uterine mucosa.PloS one · 2009Article
- Proteasome-independent degradation of HIV-1 in naturally non-permissive human placental trophoblast cells.Retrovirology · 2009Article
- Role of placenta in the vertical transmission of human immunodeficiency virus.Journal of perinatology : official journal of the California Perinatal Association · 2009Review
- Characterization of the main placental cytokine profiles from HIV-1-infected pregnant women treated with anti-retroviral drugs in France.Clinical and experimental immunology · 2007Article
- Human cytomegalovirus-caused damage to placental trophoblasts mediated by immediate-early gene-induced tumor necrosis factor-alpha.The American journal of pathology · 2002Article
- Polarized release of human cytomegalovirus from placental trophoblasts.Journal of virology · 2002Article
- Cell-to-cell contact results in a selective translocation of maternal human immunodeficiency virus type 1 quasispecies across a trophoblastic barrier by both transcytosis and infection.Journal of virology · 2001Article
- Production of interferons and beta-chemokines by placental trophoblasts of HIV-1-infected women.Infectious diseases in obstetrics and gynecology · 2001Article
- Up-regulation of CCR5 expression in the placenta is associated with human immunodeficiency virus-1 vertical transmission.The American journal of pathology · 2000Article
- The trophoblastic epithelial barrier is not infected in full-term placentae of human immunodeficiency virus-seropositive mothers undergoing antiretroviral therapy.Journal of virology · 1999Article
- Permissive cytomegalovirus infection of primary villous term and first trimester trophoblasts.Journal of virology · 1998Article
- Differential tropism and chemokine receptor expression of human immunodeficiency virus type 1 in neonatal monocytes, monocyte-derived macrophages, and placental macrophages.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whether cell-free human immunodeficiency virus type 1 (HIV-1) can productively infect placental trophoblasts (which in turn could transmit the virus into the fetal circulation) is controversial but essential to know for the evaluation of alternative routes (such as cell-mediated infection or trophoblast damage). We have addressed infection factors such as cell purity, source, culture methods, and activation states as well as virus variant and detection methods to conclusively determine the outcome of trophoblast challenge by free virus. Pure (> 99.98%) populations of trophoblasts from 11 different placentas were challenged at a multiplicity of infection (MOI) as high as 6 with five different HIV-1 variants, three of which are non-syncytium-forming, macrophage-tropic isolates from infected infants, with and without coinfection with cytomegalovirus; these preparations were monitored for productive infection for up to 3 weeks after challenge by five different criteria, the most sensitive of which were cocultivation with target cells that can detect virus at an MOI of 10(-7) and HIV DNA PCR that detects 30 virus copies per 10(5) cells. Infection was never detected. However, molecularly cloned T-cell (pNL4-3)- and macrophage (pNLAD8)-tropic provirus plasmids, when transfected into primary trophoblasts, yielded productive infections, indicating that trophoblasts do not suppress late-stage virus replication and assembly. Because of the purity of the trophoblast preparations, the extended length of the infection culture period, the number of trophoblast preparations and virus types examined, the sensitivity of the bioassays and molecular detection assays, and the observations that trophoblasts can support virus replication from provirus, the results of this study strongly argue that free virus cannot infect primary villous trophoblasts.
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