Evidence map›Paper›PMID 9260948›Full record

ArticleJournal of bacteriology1997

Gene cloning and expression and characterization of a toxin-sensitive protein phosphatase from the methanogenic archaeon Methanosarcina thermophila TM-1.

B Solow, J C Young, P J Kennelly

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Protein Ser/Thr/Tyr phosphorylation in the Archaea.The Journal of biological chemistry · 2014
    Review
  6. Article
  7. Eukaryote-like serine/threonine kinases and phosphatases in bacteria.Microbiology and molecular biology reviews : MMBR · 2011
    Review
  8. Article
  9. Posttranslational protein modification in Archaea.Microbiology and molecular biology reviews : MMBR · 2005
    Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

B SolowDepartment of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061-0308, USA.
J C Young
P J Kennelly
Virginia Tech · US

Funding

REGULATORY DOMAINS OF PROTEIN PHOSPHATASE-1R01GM055067 · NIGMS · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI KENNELLY, PETER J. · 1997 to 2004
$715k
NIGMS NIH HHS R01 GM55067
6 · The paper itself

Abstract

With oligonucleotides modelled after conserved regions within the protein-serine/threonine phosphatases (PPs) of the PP1/2A/2B superfamily, the gene for the archaeal protein phosphatase PP1-arch2 was identified, cloned, and sequenced from the methanogenic archaeon Methanosarcina thermophila TM-1. The DNA-derived amino acid sequence of PP1-arch2 exhibited a high degree of sequence identity, 27 to 31%, with members of the PP1/2A/2B superfamily such as PP1-arch1 from Sulfolobus solfataricus, PP1alpha from rats, PP2A from Saccharomyces cerevisiae, and PP2B from humans. The activity of the recombinant PP1-arch2 was sensitive to several naturally occurring microbial toxins known to potently inhibit eucaryal PP1 and PP2A, including microcystin-LR, okadaic acid, tautomycin, and calyculin A.

Indexed as

AcetatesAmino Acid SequenceCloning, MolecularGene ExpressionGenes, BacterialMethanosarcinaMolecular Sequence DataPhosphoprotein PhosphatasesPhosphoserinePhosphotyrosineRecombinant ProteinsSequence Homology, Amino AcidSubstrate SpecificityAcetatesPhosphoprotein PhosphatasesPhosphoserinePhosphotyrosineRecombinant Proteins

Identifiers

PMID9260948
PMCPMC179364
OpenAlexW1498449101

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.