ArticleProceedings of the National Academy of Sciences of the United States of America1997
Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 103 citations in OpenAlex.
- Structures of native SV2A reveal the binding mode for tetanus neurotoxin and anti-epileptic racetams.Nature communications · 2025Article
- Therapeutic Assay with the Non-toxic C-Terminal Fragment of Tetanus Toxin (TTC) in Transgenic Murine Models of Prion Disease.Molecular neurobiology · 2021Article
- Structural flexibility of the tetanus neurotoxin revealed by crystallographic and solution scattering analyses.Journal of structural biology: X · 2021Article
- Neuroanatomical tract-tracing techniques that did go viral.Brain structure & function · 2020Review
- Neuroprotective Effect of Non-viral Gene Therapy Treatment Based on Tetanus Toxin C-fragment in a Severe Mouse Model of Spinal Muscular Atrophy.Frontiers in molecular neuroscience · 2016Article
- Smuggling Drugs into the Brain: An Overview of Ligands Targeting Transcytosis for Drug Delivery across the Blood-Brain Barrier.Pharmaceutics · 2014Review
- Cargo-delivery platforms for targeted delivery of inhibitor cargos against botulism.Current topics in medicinal chemistry · 2014Review
- Fragment C of tetanus toxin: new insights into its neuronal signaling pathway.International journal of molecular sciences · 2012Review
- A conformational change of C fragment of tetanus neurotoxin reduces its ganglioside-binding activity but does not destroy its immunogenicity.Clinical and vaccine immunology : CVI · 2011Article
- Innocuous full-length botulinum neurotoxin targets and promotes the expression of lentiviral vectors in central and autonomic neurons.Gene therapy · 2011Article
- Lack of a synergistic effect of a non-viral ALS gene therapy based on BDNF and a TTC fusion molecule.Orphanet journal of rare diseases · 2011Article
- Review
- Effects of gene therapy on muscle 18S rRNA expression in mouse model of ALS.BMC research notes · 2010Article
- Tetanus toxin C-fragment: the courier and the cure?Toxins · 2010Review
- Gene transfer in the nervous system and implications for transsynaptic neuronal tracing.Expert opinion on biological therapy · 2010Review
- Fragment C of tetanus toxin, more than a carrier. Novel perspectives in non-viral ALS gene therapy.Journal of molecular medicine (Berlin, Germany) · 2010Article
- Molecular neuroanatomy's "Three Gs": a primer.Neuron · 2007Review
- Motor neuron targeting of IGF-1 attenuates age-related external Ca2+-dependent skeletal muscle contraction in senescent mice.Experimental gerontology · 2007Article
- Reduction of the ganglioside binding activity of the tetanus toxin HC fragment destroys immunogenicity: implications for development of novel tetanus vaccines.Infection and immunity · 2006Article
- How do genes regulate simple behaviours? Understanding how different neurons in the vertebrate spinal cord are genetically specified.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2006Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nontoxic proteolytic C fragment of tetanus toxin (TTC peptide) has the same ability to bind nerve cells and be retrogradely transported through a synapse as the native toxin. We have investigated its potential use as an in vivo neurotropic carrier. In this work we show that a hybrid protein encoded by the lacZ-TTC gene fusion retains the biological functions of both proteins in vivo-i.e. , retrograde transynaptic transport of the TTC fragment and beta-galactosidase enzymatic activity. After intramuscular injection, enzymatic activity could be detected in motoneurons and connected neurons of the brainstem areas. This strategy could be used to deliver a biological activity to neurons from the periphery to the central nervous system. Such a hybrid protein could also be used to map synaptic connections between neural cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.