ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Evidence that the homeodomain protein Gtx is involved in the regulation of oligodendrocyte myelination.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 72 citations in OpenAlex.
- Enhancer Dynamics and Spatial Organization Drive Anatomically Restricted Cellular States in the Human Spinal Cord.bioRxiv : the preprint server for biology · 2025Article
- Subchronic olanzapine exposure leads to increased expression of myelination-related genes in rat fronto-medial cortex.Translational psychiatry · 2017Article
- Mutations in NKX6-2 Cause Progressive Spastic Ataxia and Hypomyelination.American journal of human genetics · 2017Article
- Identification of genome-wide targets of Olig2 in the adult mouse spinal cord using ChIP-Seq.PloS one · 2017Article
- Transcriptional and Epigenetic Regulation of Oligodendrocyte Development and Myelination in the Central Nervous System.Cold Spring Harbor perspectives in biology · 2015Review
- Article
- Olig2 regulates Sox10 expression in oligodendrocyte precursors through an evolutionary conserved distal enhancer.Nucleic acids research · 2011Article
- Article
- Antisense masking reveals contributions of mRNA-rRNA base pairing to translation of Gtx and FGF2 mRNAs.The Journal of biological chemistry · 2008Article
- Separate proteolipid protein/DM20 enhancers serve different lineages and stages of development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Spatial genetic patterning of the embryonic neuroepithelium generates GABAergic interneuron diversity in the adult cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- CNS myelin paranodes require Nkx6-2 homeoprotein transcriptional activity for normal structure.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- A combinatorial network of evolutionarily conserved myelin basic protein regulatory sequences confers distinct glial-specific phenotypes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Mice lacking the Nkx6.2 (Gtx) homeodomain transcription factor develop and reproduce normally.Molecular and cellular biology · 2001Article
- The pathobiology of myelin mutants reveal novel biological functions of the MBP and PLP genes.Brain pathology (Zurich, Switzerland) · 2001Review
- rRNA-complementarity in the 5' untranslated region of mRNA specifying the Gtx homeodomain protein: evidence that base- pairing to 18S rRNA affects translational efficiency.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the patterns of postnatal brain expression and DNA binding of Gtx, a homeodomain transcription factor. Gtx mRNA accumulates in parallel with the RNAs encoding the major structural proteins of myelin, myelin basic protein (MBP), and proteolipid protein (PLP) during postnatal brain development; Gtx mRNA decreases in parallel with MBP and PLP mRNAs in the brains of myelin-deficient rats, which have a point mutation in the PLP gene. Gtx mRNA is expressed in differentiated, postmitotic oligodendrocytes but is not found in oligodendrocyte precursors or astrocytes. These data thus demonstrate that Gtx is expressed uniquely in differentiated oligodendrocytes in postnatal rodent brain and that its expression is regulated in parallel with the major myelin protein mRNAs, encoding MBP and PLP, under a variety of physiologically relevant circumstances. Using a Gtx fusion protein produced in bacteria, we have confirmed that Gtx is a sequence-specific DNA-binding protein, which binds DNA sequences containing a core AT-rich homeodomain binding site. Immunoprecipitation of labeled DNA fragments encoding either the MBP or PLP promoter regions with this fusion protein has identified several Gtx-binding fragments, and we have confirmed these data using an electrophoretic mobility shift assay. In this way we have identified four Gtx binding sites within the first 750 bp of the MBP promoter and four Gtx binding sites within the first 1. 3 kb of the PLP promoter. In addition, inspection of the PLP promoter sequence demonstrates the presence of six additional Gtx binding sites. These data, taken together, strongly suggest that Gtx is important for the function of differentiated oligodendrocytes and may be involved in the regulation of myelin-specific gene expression.
Indexed as
Identifiers
9254678PMC6573154W1497961594What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.