ArticleBiophysical journal1997
Electrorotation of single yeast cells at frequencies between 100 Hz and 1.6 GHz.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Label-free and noninvasive analysis of microorganism surface epistructures at the single-cell level.Biophysical journal · 2023Article
- A Prominent Cell Manipulation Technique in BioMEMS: Dielectrophoresis.Micromachines · 2020Review
- Combined AC-electrokinetic effects: Theoretical considerations on a three-axial ellipsoidal model.Electrophoresis · 2018Article
- Multi-frequency dielectrophoretic characterization of single cells.Microsystems & nanoengineering · 2018Article
- Membrane capacitance of thousands of single white blood cells.Journal of the Royal Society, Interface · 2017Article
- Dielectric model for Chinese hamster ovary cells obtained by dielectrophoresis cytometry.Biomicrofluidics · 2016Article
- Maxwell's mixing equation revisited: characteristic impedance equations for ellipsoidal cells.Biophysical journal · 2015Article
- Single Cell Electrical Characterization Techniques.International journal of molecular sciences · 2015Review
- Distinctive translational and self-rotational motion of lymphoma cells in an optically induced non-rotational alternating current electric field.Biomicrofluidics · 2015Article
- Assessment of 0.5 T static field exposure effect on yeast and HEK cells using electrorotation.Biophysical journal · 2013Article
- Dielectric analysis and multi-cell electrofusion of the yeast Pichia pastoris for electrophysiological studies.The Journal of membrane biology · 2012Article
- Technical aspects of measurement of cellular electromagnetic activity.European biophysics journal : EBJ · 2010Article
- Interaction between cells in dielectrophoresis and electrorotation experiments.Biomicrofluidics · 2010Article
- High-throughput cell and particle characterization using isodielectric separation.Analytical chemistry · 2009Article
- Label-free electrical quantification of the dielectrophoretic response of DNA.PMC biophysics · 2008Article
- Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments.Proceedings of the IEEE. Institute of Electrical and Electronics Engineers · 2004Article
- Particle separation by dielectrophoresis.Electrophoresis · 2002Review
- A polarization model overcoming the geometric restrictions of the laplace solution for spheroidal cells: obtaining new equations for field-induced forces and transmembrane potential.Biophysical journal · 1999Article
- Electrorotation studies of baby hamster kidney fibroblasts infected with herpes simplex virus type 1.Biophysical journal · 1999Article
- Dielectric single particle spectroscopy for measurement of dispersion.Medical & biological engineering & computing · 1999Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The determination of complete electrorotation spectra of living cells has been made possible by the development of a quadrature generator and an electrode assembly that span the frequency range between 100 Hz and 1.6 GHz. Multiple spectra of single cells of the yeast Saccharomyces cerevisiae have been measured at different medium conductivities ranging from 0.7 to 550 microS cm-1. A spherical four-shell model was applied that simulated the experimental data well and disclosed the four-layer structure of the cell envelope attributed to the plasma membrane, the periplasmic space, and a thick inner and a thin outer wall region. Below 10 kHz an additional rotation effect was found, which changed its direction depending on the ionic strength of the medium. This is supposed to be connected with properties of the cell surface and its close vicinity. From the four-shell simulation the following physical properties of cell compartments could be derived: specific capacitance of plasma membrane (0.76 microF cm-2), periplasmic space (0.5 microF cm-2), and outer wall region (0.1 microF cm-2). The conductivity of cytoplasm, plasma membrane, and inner wall region were found to vary with medium ionic strength from 9 to 12 mS cm-1, 5.8 nS cm-1 to approximately 50 nS cm-1, and 6 microS cm-1 to 240 microS cm-1, respectively.
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