ArticleBiophysical journal1997
Single-molecule microscopy on model membranes reveals anomalous diffusion.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 179 papers.
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Who cites it
179 citing papers in PubMed.
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- The kinetics and mobility of a ParA ATPase drive carboxysome distribution inbioRxiv : the preprint server for biology · 2026Article
- Revealing plasma membrane protein dynamics in living plant cells with single-molecule tracking.Journal of experimental botany · 2025Article
- Ligand Separation on Nanoconstructs Affects Targeting Selectivity to Protein Dimers on Cell Membranes.Nano letters · 2024Article
- Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.EMBO reports · 2023Article
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- Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements.Frontiers in neuroinformatics · 2023Article
- Classification-based motion analysis of single-molecule trajectories using DiffusionLab.Scientific reports · 2022Article
- Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5.Biophysical reports · 2022Article
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- Single molecule tracking of bacterial cell surface cytochromes reveals dynamics that impact long-distance electron transport.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- When Super-Resolution Localization Microscopy Meets Carbon Nanotubes.Nanomaterials (Basel, Switzerland) · 2022Review
- Emerin self-assembly and nucleoskeletal coupling regulate nuclear envelope mechanics against stress.Journal of cell science · 2022Article
- ATP-responsive biomolecular condensates tune bacterial kinase signaling.Science advances · 2022Article
- Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue.Disease models & mechanisms · 2022Article
- Impact of neurite alignment on organelle motion.Journal of the Royal Society, Interface · 2022Article
- Subdiffusive-Brownian crossover in membrane proteins: a generalized Langevin equation-based approach.Biophysical journal · 2021Article
119 more citing papers are in PubMed but not listed here.
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The lateral mobility of lipids in phospholipid membranes has attracted numerous experimental and theoretical studies, inspired by the model of Singer and Nicholson (1972. Science, 175:720-731) and the theoretical description by Saffman and Delbrück (1975. Proc. Natl. Acad. Sci. USA. 72:3111-3113). Fluorescence recovery after photobleaching (FRAP) is used as the standard experimental technique for the study of lateral mobility, yielding an ensemble-averaged diffusion constant. Single-particle tracking (SPT) and the recently developed single-molecule imaging techniques now give access to data on individual displacements of molecules, which can be used for characterization of the mobility in a membrane. Here we present a new type of analysis for tracking data by making use of the probability distribution of square displacements. The potential of this new type of analysis is shown for single-molecule imaging, which was employed to follow the motion of individual fluorescence-labeled lipids in two systems: a fluid-supported phospholipid membrane and a solid polymerstabilized phospholipid monolayer. In the fluid membrane, a high-mobility component characterized by a diffusion constant of 4.4 microns2/s and a low-mobility component characterized by a diffusion constant of 0.07 micron2/s were identified. It is proposed that the latter characterizes the so-called immobile fraction often found in FRAP experiments. In the polymer-stabilized system, diffusion restricted to corrals of 140 nm was directly visualized. Both examples show the potentials of such detailed analysis in combination with single-molecule techniques: with minimal interference with the native structure, inhomogeneities of membrane mobility can be resolved with a spatial resolution of 100 nm, well below the diffraction limit.
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