ArticleBiophysical journal1997
Formation of stable polypeptide monolayers at interfaces: controlling molecular conformation and orientation.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Elucidating the role of carrier proteins in cytokine stabilization within double emulsion-based polymeric nanoparticles.Bioengineering & translational medicine · 2025Article
- How Permanent Are the Permanent Macrodipoles of Anthranilamide Bioinspired Molecular Electrets?Journal of the American Chemical Society · 2024Article
- Highly selective and antifouling electrochemical biosensors for sensitive MicroRNA assaying based on conducting polymer polyaniline functionalized with zwitterionic peptide.Analytical and bioanalytical chemistry · 2021Article
- Multifaceted aspects of charge transfer.Physical chemistry chemical physics : PCCP · 2020Article
- Structure and Conformation of Wild-Type Bacterial Lipopolysaccharide Layers at Air-Water Interfaces.Biophysical journal · 2019Article
- Two-dimensional sum-frequency generation reveals structure and dynamics of a surface-bound peptide.Journal of the American Chemical Society · 2014Article
- On-surface assembly of coiled-coil heterodimers.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Sequence, structure, and function of peptide self-assembled monolayers.Journal of the American Chemical Society · 2012Article
- A kinetic model for beta-amyloid adsorption at the air/solution interface and its implication to the beta-amyloid aggregation process.The journal of physical chemistry. B · 2009Article
- Tethered bilayer lipid membranes studied by simultaneous attenuated total reflectance infrared spectroscopy and electrochemical impedance spectroscopy.The journal of physical chemistry. B · 2007Article
- Secondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopy.The Biochemical journal · 2005Article
- Atomic force bio-analytics of polymerization and aggregation of phycoerythrin-conjugated immunoglobulin G molecules.Molecular immunology · 2004Article
- Molecular dynamics simulations on the first two helices of Vpu from HIV-1.Biophysical journal · 2003Article
- Stable self-assembly of a protein engineering scaffold on gold surfaces.Protein science : a publication of the Protein Society · 2002Article
- Self-assembly of the hydrophobin SC3 proceeds via two structural intermediates.Protein science : a publication of the Protein Society · 2002Article
- Peptide interfacial adsorption is kinetically limited by the thermodynamic stability of self association.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Polarization-modulated FTIR spectroscopy of lipid/gramicidin monolayers at the air/water interface.Biophysical journal · 1999Article
- Critical role of conserved proline residues in the transmembrane segment 4 voltage sensor function and in the gating of L-type calcium channels.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular self-organization and structural properties of peptide assemblies at different interfaces, using either amphipathic or hydrophobic polypeptide helices, is described. The two peptides under investigation form stable monolayers on the water surface under the conservation of their molecular conformation, as studied by circular dichroism and polarization-modulation Fourier transform infrared (FTIR) spectroscopy. Using surface plasmon resonance and reflection-absorption FTIR, we show that such molecular layers can be transferred unaltered to solid substrates. Most importantly, the molecular orientation of the hydrophobic helices on solid supports such as gold can be controlled by choosing a particular procedure for the layer formation. The helices were oriented parallel to the interface in Langmuir-Blodgett monolayers, and perpendicular to the interface in self-assembled monolayers. Our reflection-absorption FTIR measurements have delivered for the first time direct experimental evidence for the molecular conformation and orientation of pure peptide monolayers. Suitable reference spectra of polypeptides with defined conformation and orientation are necessary to use this technique for the determination of the molecular orientation of peptides in monomolecular films. We have solved the problem for alpha-helical polypeptides by using bacteriorhodopsin as a reference in combination with synthetic alpha-helices of defined interfacial orientation. The present study shows the possibility of constructing immobilized peptide monolayers with predefined macroscopic properties and molecular structure by choosing the proper polypeptide amino acid sequence, the technique used for layer formation, and the supporting surface properties.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.