ArticleThe EMBO journal1997
Identification of a human protein that recognizes the 3' splice site during the second step of pre-mRNA splicing.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- Alternative Splicing of ALS Genes: Misregulation and Potential Therapies.Cellular and molecular neurobiology · 2020Review
- Role of FET proteins in neurodegenerative disorders.RNA biology · 2016Review
- ALS mutations in TLS/FUS disrupt target gene expression.Genes & development · 2015Article
- Increased Serine-Arginine (SR) Protein Phosphorylation Changes Pre-mRNA Splicing in Hypoxia.The Journal of biological chemistry · 2015Article
- Polypyrimidine tract binding protein inhibits IgM pre-mRNA splicing by diverting U2 snRNA base-pairing away from the branch point.RNA (New York, N.Y.) · 2014Article
- NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins.Nucleic acids research · 2014Article
- Ewing sarcoma protein: a key player in human cancer.International journal of cell biology · 2013Review
- The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing.Genes & development · 2010Article
- TDP-43 and FUS/TLS: emerging roles in RNA processing and neurodegeneration.Human molecular genetics · 2010Review
- The TET family of proteins: functions and roles in disease.Journal of molecular cell biology · 2009Review
- Distinct activities of the DExD/H-box splicing factor hUAP56 facilitate stepwise assembly of the spliceosome.Genes & development · 2008Article
- Retroviral splicing suppressor sequesters a 3' splice site in a 50S aberrant splicing complex.Molecular and cellular biology · 2005Article
- Template-dependent incorporation of 8-N3AMP into RNA with bacteriophage T7 RNA polymerase.RNA (New York, N.Y.) · 2004Article
- Article
- A single polypyrimidine tract binding protein (PTB) binding site mediates splicing inhibition at mouse IgM exons M1 and M2.RNA (New York, N.Y.) · 2004Article
- p54(nrb) associates with the 5' splice site within large transcription/splicing complexes.The EMBO journal · 2004Article
- Convergence of RNA cis elements and cellular polyadenylation factors in the regulation of human cytomegalovirus UL37 exon 1 unspliced RNA production.Journal of virology · 2003Article
- Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Functional selection of splicing enhancers that stimulate trans-splicing in vitro.RNA (New York, N.Y.) · 2001Article
- Interaction of the yeast DExH-box RNA helicase prp22p with the 3' splice site during the second step of nuclear pre-mRNA splicing.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate splicing of precursor mRNAs (pre-mRNAs) requires recognition of the 5' and 3' splice sites at the intron boundaries. Interactions between several splicing factors and the 5' splice site, which occur prior to the first step of splicing, have been well described. In contrast, recognition of the 3' splice site, which is cleaved during the second catalytic step, is poorly understood, particularly in higher eukaryotes. Here, using site-specific photo-crosslinking, we find that the conserved AG dinucleotide at the 3' splice site is contacted specifically by a 70 kDa polypeptide (p70). The p70-3' splice site crosslink has kinetics and biochemical requirements similar to those of splicing, was detected only in the mature spliceosome and occurs subsequent to the first step. Thus, p70 has all the properties expected of a factor that functionally interacts with the 3' splice site during the second step of splicing. Using antisera to various known splicing factors, we find that p70 corresponds to a previously reported 69 kDa protein of unknown function associated with the Sm core domain of spliceosomal small nuclear ribonucleoproteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.