ArticleMolecular biology of the cell1997
Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 170 citations in OpenAlex.
- A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.PloS one · 2022Article
- Type V myosin focuses the polarisome and shapes the tip of yeast cells.The Journal of cell biology · 2021Article
- Haspin regulates Ras localization to promote Cdc24-driven mitotic depolarization.Cell discovery · 2020Article
- Integrated control of formin-mediated actin assembly by a stationary inhibitor and a mobile activator.The Journal of cell biology · 2018Article
- Role of Mitochondrial Retrograde Pathway in Regulating Ethanol-Inducible Filamentous Growth in Yeast.Frontiers in physiology · 2017Article
- The final cut: cell polarity meets cytokinesis at the bud neck in S. cerevisiae.Cellular and molecular life sciences : CMLS · 2016Review
- Proteins interacting with mitochondrial ATP-dependent Lon protease (MAP1) in Magnaporthe oryzae are involved in rice blast disease.Molecular plant pathology · 2015Article
- A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis.Molecular biology of the cell · 2015Article
- A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip.The Journal of cell biology · 2015Article
- The long and viscous road: uncovering nuclear diffusion barriers in closed mitosis.PLoS computational biology · 2014Article
- Linking genetics to structural biology: complex heterozygosity screening with actin alanine scan alleles identifies functionally related surfaces on yeast actin.G3 (Bethesda, Md.) · 2014Article
- Article
- The yeast Sks1p kinase signaling network regulates pseudohyphal growth and glucose response.PLoS genetics · 2014Article
- Here, there, everywhere. mRNA localization in budding yeast.RNA biology · 2014Review
- Deconstructing formin-dependent actin cable assembly.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Different polarisome components play distinct roles in Slt2p-regulated cortical ER inheritance in Saccharomyces cerevisiae.Molecular biology of the cell · 2013Article
- Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation.The Journal of cell biology · 2013Article
- Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cdc42p regulation of the yeast formin Bni1p mediated by the effector Gic2p.Molecular biology of the cell · 2012Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
26 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
Funding
Abstract
A search for Saccharomyces cerevisiae proteins that interact with actin in the two-hybrid system and a screen for mutants that affect the bipolar budding pattern identified the same gene, AIP3/BUD6. This gene is not essential for mitotic growth but is necessary for normal morphogenesis. MATa/alpha daughter cells lacking Aip3p place their first buds normally at their distal poles but choose random sites for budding in subsequent cell cycles. This suggests that actin and associated proteins are involved in placing the bipolar positional marker at the division site but not at the distal tip of the daughter cell. In addition, although aip3 mutant cells are not obviously defective in the initial polarization of the cytoskeleton at the time of bud emergence, they appear to lose cytoskeletal polarity as the bud enlarges, resulting in the formation of cells that are larger and rounder than normal. aip3 mutant cells also show inefficient nuclear migration and nuclear division, defects in the organization of the secretory system, and abnormal septation, all defects that presumably reflect the involvement of Aip3p in the organization and/or function of the actin cytoskeleton. The sequence of Aip3p is novel but contains a predicted coiled-coil domain near its C terminus that may mediate the observed homo-oligomerization of the protein. Aip3p shows a distinctive localization pattern that correlates well with its likely sites of action: it appears at the presumptive bud site prior to bud emergence, remains near the tips of small bund, and forms a ring (or pair of rings) in the mother-bud neck that is detectable early in the cell cycle but becomes more prominent prior to cytokinesis. Surprisingly, the localization of Aip3p does not appear to require either polarized actin or the septin proteins of the neck filaments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.