ArticleMolecular biology of the cell1997
An N-terminal fragment of titin coupled to green fluorescent protein localizes to the Z-bands in living muscle cells: overexpression leads to myofibril disassembly.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Effect of ACTN3 Gene Doping on Skeletal Muscle Performance.American journal of human genetics · 2018Pooled it
- Four New Muscle Myosin II Binding Properties of Titin: Implications for Myofibrillogenesis.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Inhibitors of the ubiquitin proteasome system block myofibril assembly in cardiomyocytes derived from chick embryos and human pluripotent stem cells.Cytoskeleton (Hoboken, N.J.) · 2021Article
- Comparative analysis of the transcriptomes of EDL, psoas, and soleus muscles from mice.BMC genomics · 2020Article
- Real-time visualization of titin dynamics reveals extensive reversible photobleaching in human induced pluripotent stem cell-derived cardiomyocytes.American journal of physiology. Cell physiology · 2020Article
- Resolving titin's lifecycle and the spatial organization of protein turnover in mouse cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Expression of myotilin during chicken development.Anatomical record (Hoboken, N.J. : 2007) · 2014Article
- Heavy and light roles: myosin in the morphogenesis of the heart.Cellular and molecular life sciences : CMLS · 2013Review
- Titin visualization in real time reveals an unexpected level of mobility within and between sarcomeres.The Journal of cell biology · 2011Article
- Assembly and dynamics of myofibrils.Journal of biomedicine & biotechnology · 2010Review
- Dynamics of Z-band based proteins in developing skeletal muscle cells.Cell motility and the cytoskeleton · 2005Article
- Assembly of the giant protein projectin during myofibrillogenesis in Drosophila indirect flight muscles.BMC cell biology · 2004Article
- D-Titin: a giant protein with dual roles in chromosomes and muscles.The Journal of cell biology · 2000Article
- Cardiomyocyte cytoskeleton and myofibrillogenesis in healthy and diseased heart.Heart failure reviews · 2000Review
- Nspl1, a new Z-band-associated protein.Journal of muscle research and cell motility · 1999Article
- Thick filament assembly occurs after the formation of a cytoskeletal scaffold.Journal of muscle research and cell motility · 1999Article
- Z-line structural diversity in frog single muscle fiber in the passive state.Journal of muscle research and cell motility · 1999Article
- The NH2 terminus of titin spans the Z-disc: its interaction with a novel 19-kD ligand (T-cap) is required for sarcomeric integrity.The Journal of cell biology · 1998Article
- Expression of sarcomeric proteins and assembly of myofibrils in the putative myofibroblast cell line BHK-21/C13.Journal of muscle research and cell motility · 1998Article
- Human autoantibodies reveal titin as a chromosomal protein.The Journal of cell biology · 1998Article
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5 authors.
Funding
Abstract
Cultures of nonmuscle cells, skeletal myotubes, and cardiomyocytes were transfected with a fusion construct (Z1.1GFP) consisting of a 1.1-kb cDNA (Z1.1) fragment from the Z-band region of titin linked to the cDNA for green fluorescent protein (GFP). The Z1.1 cDNA encodes only 362 amino acids of the approximately 2000 amino acids that make up the Z-band region of titin; nevertheless, the Z1.1GFP fusion protein targets the alpha-actinin-rich Z-bands of contracting myofibrils in vivo. This fluorescent fusion protein also localizes in the nascent and premyofibrils at the edges of spreading cardiomyocytes. Similarly, in transfected nonmuscle cells, the Z1.1GFP fusion protein localizes to the alpha-actinin-containing dense bodies of the stress fibers in vivo. A dominant negative phenotype was also observed in living cells expressing high levels of this Z1.1GFP fusion protein, with myofibril disassembly occurring as titin-GFP fragments accumulated. These data indicate that the Z-band region of titin plays an important role in maintaining and organizing the structure of the myofibril. The Z1.1 cDNA was derived from a chicken cardiac lambda gt11 expression library, screened with a zeugmatin antibody. Recent work has suggested that zeugmatin is actually part of the N-terminal region of the 81-kb titin cDNA. A reverse transcriptase polymerase chain reaction using a primer from the distal end (5' end) of the Z1.1 zeugmatin cDNA and a primer from the nearest known proximal (3' end) chicken titin (also called connectin) cDNA resulted in a predicted 0.3-kb polymerase chain reaction product linking the two known chicken titin cDNAs to each other. The linking region had a 79% identity at the amino acid level to human cardiac titin. This result and a Southern blot analysis of chicken genomic DNA hybridized with Z1.1 add further support to our original suggestion that zeugmatin is a proteolytic fragment from the N-terminal region of titin.
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